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冰草属(Agropyron Gaertn.)植物遗传多样性取样策略基于醇溶蛋白的研究
引用本文:车永和,李立会,何蓓如.冰草属(Agropyron Gaertn.)植物遗传多样性取样策略基于醇溶蛋白的研究[J].植物遗传资源学报,2004,5(3):216-221.
作者姓名:车永和  李立会  何蓓如
作者单位:1. 中国农业科学院作物品种资源研究所,北京,100081;西北农林科技大学农学院,陕西杨凌,712100
2. 中国农业科学院作物品种资源研究所,北京,100081
3. 西北农林科技大学农学院,陕西杨凌,712100
摘    要:对冰草属(Agropyron Gaertn.)植物的5个种22个居群分8个取样梯度,分别随机取3、6、9、12、15、18、21、24粒种子的混合样进行醇溶蛋白的A-PAGE分析,结果发现:平均Simpson指数依取样量的增加表现增大,以3粒种子0.922表现最低,以21粒0.933表现最高,变异系数(CV%)为0.41.醇溶蛋白谱带数依所提取的混合样的籽粒数目发生显著变化,冰草属内5个种表现趋势相同,22个居群不同取样整体平均以3粒种子提取的醇溶蛋白谱带数18.38为最少,以18粒种子提取的醇溶蛋白谱带28.24为最多,CV%为13.21.取样量达到12粒以上醇溶蛋白谱带差异不明显(P=0.01),图谱表现基本一致.建议在利用生化指纹进行冰草属居群间及种间的遗传多样性研究中,混合取样量最低应保持在12个个体及以上方能代表居群整体,反映居群的整体遗传特性.

关 键 词:冰草属  居群  醇溶蛋白  取样
修稿时间:2004年3月22日

Sampling Strategy for Genetic Diversity in Agropyron Gaertn.Based on Gliadin
CHE Yong-he,LI Li-hui,HE Bei-ru.Sampling Strategy for Genetic Diversity in Agropyron Gaertn.Based on Gliadin[J].Journal of Plant Genetic Resources,2004,5(3):216-221.
Authors:CHE Yong-he  LI Li-hui  HE Bei-ru
Institution:CHE Yong-he 1,2,LI Li-hui1,HE Bei-ru2
Abstract:An improved ph3.2 A-PAGE method was utilized to analyze the gliadin banding pattern with eight sampling methods 22 populations of 5 species in Agropyron Gaertn. The results showed that simpson indexes increased along with the seed number in the bulked seed samples increasing. The whole gliadin banding patterns obviously changed among different seed samples.On the whole average, the most gliadin number was 28.24 when seed number were 18, and the least was 18.38 when bulked seed samples were 3 seeds. CV% of the gliadin number was 13.21 meanwhile, the gliadin banding patterns were relatively uniform when bulked seed samples surpassed 12 (included 12) seeds. Therefore, bulked seed samples above 12 could be chosen to represent whole populations based on the genetic diversity of gliadin.
Keywords:A-PAGE
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