小粒野生稻导入系对稻飞虱的抗性鉴定与分析

稻飞虱是水稻生产最严重的害虫之一。野生稻拥有丰富的抗虫基因资源,导入系是鉴定和利用野生稻有利基因的有效途径。本研究通过对371份小粒野生稻导入系进行抗褐飞虱和白背飞虱接虫鉴定,分别筛选出了11份抗、72份中抗褐飞虱的材料和7份抗、45份中抗白背飞虱的材料,其中有5份材料兼抗褐飞虱和白背飞虱,这是从小粒野生稻中鉴定出抗白背飞虱材料的首次报道。通过对2份抗性导入系材料与感虫亲本杂交构建的F1和F2群体的抗虫鉴定和分析表明:K41对褐飞虱和白背飞虱的抗性受2对显性抗虫基因通过互补作用所控制;P114对褐飞虱和白背飞虱的抗性都是由1对主效的隐性基因控制。这些结果必将有利于小粒野生稻抗稻飞虱的基因定位和育种利用。

Identification and Genetic Analysis of Resistance to Planthopper in Substitution Lines from Oryza minuta

Planthopper is one of the most destructive insect pests of rice. Wild species of rice are a valuable source of resistance genes for developing resistant cultivars. Substitution lines population was effectively used in identification and utilization of favorable genes from wild rice. In the present study a substitution lines population originating from the cross of Oryza sativa and Oryza minuta was screened for obtaining resistant resources to two brown planthopper(BPH) and whitebacked planthopper(WBPH). Among 371 substitution lines, 11 and 7 were identified to be resistant to BPH and WBPH respectively, 5 were identified to be resistant to BPH and WBPH. This is the first report of WBPH resistant materials from Oryza minuta. For genetic analysis of the resistance, 2 resistant substitution lines, named K41 and P114, were crossed with indica rice variety IR24, Gui 99, 1025. And it was found that the plants of F1 from K41 were resistant to BPH and WBPH, but the plants of F1 from P114 were susceptible to the disease. Segregation of resistant and susceptible progenies fitted a 1:3 ratio in two F2 populations derived from P114, while segregation of resistant and susceptible progenies fitted a 9:7 ratio in two F2 populations derived from K41. The results suggested that the resistance originated from resistant source P114 was controlled by one recessive gene, and the resistance originated from resistant source K41 was controlled by two dominant genes interactions. These results here will be useful to breeding and mapping of resistance to BPH and WBPH gene from Oryza minuta.