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| 中国扁蓿豆遗传多样性的ISSR分析 | |
| 引用本文： | 李志勇,李鸿雁,石凤翎,王小丽,赵成贵,师文贵,蔡丽艳.中国扁蓿豆遗传多样性的ISSR分析[J].植物遗传资源学报,2012,13(1):48-51. |
| 作者姓名： | 李志勇 李鸿雁 石凤翎 王小丽 赵成贵 师文贵 蔡丽艳 |
| 作者单位： | 1. 中国农业科学院草原研究所/农业部沙尔沁牧草资源重点野外科学观测试验站,呼和浩特,010010 2. 内蒙古农业大学生态环境学院,呼和浩特,010019 3. 中国农业科学院郑州果树研究所,郑州,450009 4. 内蒙古乌兰察布市凉城县畜牧局,凉城,013700 |
| 基金项目： | 农业部农作物种质资源保护与利用项目（NB2010-2130135-32）;牧草种质资源保护项目（2010-43）;中央级公益性科研院所基本科研业务费专项 |
| 摘 要： | 利用ISSR标记,对来源于中国7个省(市)的44个扁蓿豆居群的亲缘关系进行了分析。从80条ISSR引物中筛选出多态性强、重复性好的16条引物,对44个扁蓿豆居群基因组DNA进行扩增,共扩增出133条谱带,平均每个引物扩增出8.31条带,其中多态性带115条,多态性位点百分率为87.08%。扁蓿豆种质间遗传相似系数变化范围在0.436～0.908之间。利用UPGMA聚类分析,44个扁蓿豆居群被划分为8个类群,与地理来源有较高的相关性。 |
| 关 键 词： | 扁蓿豆 种质资源 亲缘关系 ISSR |
| 修稿时间： | 8/19/2011 3:38:30 PM |
Genetic Diversity Analysis of Medicago ruthenica by ISSRs in China |
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| LI Zhi-yong , LI Hong-yan , SHI Feng-ling , WANG Xiao-li , ZHAO Cheng-gui , SHI Wen-gui , CAI Li-yan.Genetic Diversity Analysis of Medicago ruthenica by ISSRs in China[J].Journal of Plant Genetic Resources,2012,13(1):48-51. | |
| Authors: | LI Zhi-yong LI Hong-yan SHI Feng-ling WANG Xiao-li ZHAO Cheng-gui SHI Wen-gui CAI Li-yan |
| Institution: | Zhengzhou Fruit Research Institute of The Chinese Academy of Agriculture Science,Animal Husbandry Burean Liang Cheng County Inner Mongolia,,Grassland Research Institute of The Chinese Academy of Agriculture Science |
| Abstract: | The genetic relationship of M. ruthenica populations were analyzed from seven provinces of China by ISSR marker. 16 primers with strong polymorphism and good repeatability were screened from 80 ISSR primers. Amplification of 44 populations M. ruthenica genomic DNA ,133 bands were amplified, an average of each primer amplified 8.31 bands, of which (115 bands)87.08% were polymorphic. The genetic dissimilarity coefficients of M.ruthenica germplasm ranged from 0.436 to 0.908. 44 populations M. ruthenica germplasm were divided into eight groups with the higher correlation of geography resource by UPGMA cluster analysis. |
| Keywords: | Medicago ruthenica Germplasm Genetic Relationship ISSR |
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