小麦TaLEA1基因的表达特征及抗逆功能验证

我国土壤盐碱化日益严重,对我国的粮食安全造成了严重威胁,因此耐盐基因挖掘对作物耐盐育种非常重要。许多研究表明胚胎发育晚期丰富蛋白(LEA)在植物应对非生物胁迫中发挥积极作用。本研究以小麦TaLEA1基因为研究对象,分析了其表达蛋白的理化性质及基因表达模式,并通过在拟南芥中过表达,分析Ta LEA1基因的抗逆功能。结果表明,TaLEA1基因的表达蛋白属于第3组LEA蛋白,是稳定的亲水蛋白,富含α-螺旋、β-转角等结构。Ta LEA1基因在小麦根、茎、叶、花、种子等不同组织中均有表达,盐胁迫条件诱导其高表达。在拟南芥中过表达TaLEA1基因,显著提高了盐胁迫下转基因拟南芥的种子萌发率、根长及盐和旱胁迫下的叶绿素含量。本研究结果为LEA基因抗逆机理的研究和耐盐基因的挖掘提供了重要信息。

Expression Analysis of Wheat TaLEA1 Gene and Its Functional Analysis in Arabidopsis

The degree of soil salinization in China is becoming more serious in cultivated land,which poses a major threat to China's food security in future.Identifying novel salt-tolerant genes and understanding their functions in salt stress adaptation will provide us the basis for effective engineering strategies to improve crop stress tolerance.Many studies have shown that late embryogenesis abundant protein (LEA) plays a positive role in the process of plant responses to abiotic stress.In this study,we isolated TaLEA1 gene and analyzed its expression pattern,physical and chemical properties of LEA1 protein as well as its function by overexpressing in Arabidopsis Heynh..The results showed that TaLEA1 belonged to the third group LEA proteins based on amino acid sequence analysis.LEA1 was stably hydrophilic protein and rich in α-helix and 3-turn,which provided structure foundation for its response to stress.TaLEA1 expressions were detected in wheat root,stem,leaf,flower and seed,and high salt stress could induce TaLEA1 expression.Overexpression of TaLEA1 confers salt and drought tolerance in Arabidopsis thaliana with higher seed germination rate,longer root length and higher chlorophyll content than the wild-type.The results and achievements in this study will provide the gene associated with salt tolerance with great potential for wheat improvement and the theoretically basis for deciphering the salt tolerance mechanism of the candidate gene.