小麦胁迫相关基因W1的克隆及表达模式分析

应用噬菌体原位杂交技术从干旱胁迫诱导的小麦cDNA文库中克隆到一个胁迫诱导的基因片段别。删全长cDNA为901bp，其中，编码区长498bp，编码166个氨基酸。Southern杂交表明，W1是一个低拷贝基因。RT—PCR结果表明，W1受干旱、低温的诱导，但不受高盐的诱导。氨基酸序列分析发现W1有一个USP保守区（pfam00582）。同源性分析发现W1与一个水稻胁迫诱导蛋白（NM_001061239）的同源性为83％，但该类蛋白的功能尚无报道。肼是小麦第1个被克隆的胁迫相关蛋白基因，该基因的克隆有助于阐明小麦的抗逆机制，并为今后培育抗逆性小麦品种提供候选基因。

Cloning and Expression Analysis of a Stress-related Gene W1 in Wheat

A putative stress-induced gene,W1,was cloned from the cDNA library of drought-treated wheat seedlings by phage hybridization in situ.The full-length cDNA of W1 consists of 901bp and contains a 498bp open reading frame(ORF) encoding a 166 amino acid protein.Southern blot analysis indicated that W1 was a low-copy gene.RT-PCR analysis revealed that the expression of W1 was upregulated by drought and cold.Amino acid sequence analysis discovered that W1 had a conserved region of USP(pfam00582).Phylogenetic analysis showed that W1 was 83% identical to a rice stress-induced protein(NM_001061239).However,genes of the group involved in putative stress responses were not reported.W1 is the first isolated USP gene in wheat,which promote to clarify the stress-resistant mechanism and provide a candidate gene for improving wheat stress-tolerant cultivars.