凉山州马铃薯栽培品种的遗传多样性分析

利用SSR分子标记技术对凉山州马铃薯地方品种、自育品种和各地育成品种共计60份进行了遗传多样性分析，筛选出16对有效SSR引物。采用筛选的16对引物在供试材料中共检测到68个位点，其中多态性位点42个占总扩增位点的61.76%，平均PIC值为0.4717。通过NTSYSpc 2.0对所有材料间的遗传距离进行计算，平均遗传距离在2.43~3.96之间。使用MEGA4分析软件将供试材料分成3大类，2个凉山州地方品种在聚类图中最先被分离出来。利用PoPGEN将凉山州地方种和其余材料分成两类,。上述结果表明凉山州地方种与试验中其他品种的遗传基础差异较大，是较好的资源材料。该研究为凉山州地方马铃薯品种在今后育种中的应用提供了部分参考依据。

Analyzing of genetic diversity between liangshan potato landraces, varieties bred by xichang college and varieties bred by other place

Applying SSR molecular marker technology to analysis genetic diversity for 60 potato cultivars which contained liangshan landraces, varieties bred by xichang college and varieties bred by other place. We had Screened out 16 effective SSR primers. 68 loci had been found using those markers in the test materials. There were 42 polymorphic loci which accounted for 61.76%. The average value of PIC was 0.4717. The genetic distance among all materials had been calculated by NTSYSpc2.0, the average genetic distance between 2.43 to 3.96. The test materials had been divided into 3 categories through MEGA4 analysis software and in the dendrogram two Liangshan landraces was first separated out. The Liangshan landraces and other materials had been classified into two groups by PoPGEN. Those results indicated definite difference between Liangshan landraces and other materials in the genetic basis. So the Liangshan landraces are useful resource for breeding. This study provides some partly reference for application of Liangshan landraces potatoes in the further breeding.