基于SSR标记的山东省小麦DNA指纹图谱的构建

本文以山东省41份小麦种质资源为材料，使用46对引物，通过SSR技术对其进行了DNA指纹数据库的构建。结果显示，所采用的46对引物在本实验材料中共检测到69个等位基因，有较好的多态性。利用NTSYS进行UPGMA聚类分析后，发现这41种材料在遗传相似系数0.68为阀值处可以分为3个类群。实验证明该方法能够分辨各个种质间的亲缘关系，能够较好满足小麦DNA指纹数据库的构建要求，对山东省小麦遗传资源的收集、保存、分类、评价、核心种质的建立等研究工作提供理论依据。

Establishiment of DNA Fingerprinting for Wheat in Shandong Province by SSR Markers

In this paper, 41 unique wheat cultivars of Shandong Province were collected to construct DNA fingerprint database by SSR technique. The results showed that 69 alleles were detected with considerable polymorphism by 46 pairs of primers employed in the experimental design. For UPGMA cluster analysis using NTSYS, it was found that 41 kinds of materials could be divided into three groups with the threshold at genetic similarity of 0.68. This study indicates that (SSR technique) could effectively detect the genetic relationships among the various wheat cultivars, and it could meet the requirements for the construction of DNA fingerprint database for wheat, providing theory basis for collecting, storing, classifying, evaluating, and establishment of the core germplasm of wheat cultivars genetic resources of Shandong Province.