油菜种子类胡萝卜素总量测定方法的研究

类胡萝卜素是植物中最重要的一类色素,具有抗氧化、预防心血管疾病和癌症、延缓衰老等生物学功能,与人类健康关系密切.培育高类胡萝卜素油菜品种能有效地提升菜籽油的营养保健价值和稳定性,是今后油菜品质育种的一个新方向.研究建立快速、准确和简便的油菜种子类胡萝卜素总量测定方法,是发掘高类胡萝卜素油菜种质资源和开展高类胡萝卜素油菜育种的前提条件.本实验研究了不同提取溶剂、时间、料液比等因素对油菜种子中类胡萝卜素的提取效果和测定结果的影响,确定了最佳的提取和测定条件.建立了快速、准确和简便的油菜种子类胡萝卜素总量测定方法,并测定了30份不同类型油菜种质资源种子中类胡萝卜素总量,为进一步发掘高类胡萝卜素油菜种质资源和开展高类胡萝卜素油菜育种创造了条件.

Study on Mensuration of Total Carotenoids Content in Rapeseeds

Carotenoids is one of the most important member of plant pigments and has diverse functions such as antioxidant,preventing cardiovascular disease and cancer,antiaging.It is a new trend to breed rapeseed cultivar with high carotenoid(HC) content,since HC canola oil may enhance nutrition value and stability.Carotenoids was extracted from rapeseeds in this experiment,and the influence of different solvents,time,the ration of rapeseeds on extraction process was studied,and the optimum condition was obtained.We set up a fast,accurate,simple and convienient method to measure the total carotenoids content in rapeseeds,and the contents of 30 accession of rapeseed germplasm were determined by using this method.The established method provide a good base for the exploiting of high carotenoid rape germplasm and quality breeding for the high carotenoid rape.