大豆异黄酮合成途径相关基因差异表达分析

大豆异黄酮是一种应用广泛、具有医用和保健功能的活性物质。为揭示异黄酮合成途径相关基因表达差异,本研究采用实时定量PCR技术分析相关基因在不同大豆品种、发育时期及组织部位的表达。结果发现,苯丙氨酸解氨酶基因PAL、肉桂酸羟化酶基因C4H、香豆酸辅酶A连接酶基因4CL在高异黄酮品种中豆27 R2期叶片中的表达量显著高于低异黄酮品种楚秀;查尔酮合成酶基因CHS、异黄酮合成酶基因IFS在中豆27 R8期子粒中的表达量显著高于楚秀;细胞色素还原酶基因CPR在中豆27 R7期叶片与子粒的表达量与楚秀相比显著降低。这些差异表达的基因可能是形成大豆品种异黄酮含量高低的重要原因。

Differential Expression Analysis of Genes Associated with Isoflavone Synthesis in Soybean (Glycine max)

The soybean isoflavone is a kind of widely used active materials which has medical and healthcare functions. To analyze the differential expression patterns of genes associated with isoflavone synthesis in soybean, seven related genes were studied at different developmental stages and organs in different varieties by real-time quantitative PCR method. The results showed that the expression levels of phenylalanine ammonialyase PAL, cinnamate-4-hydroxylase C4H and 4-coumarate: coenzyme A ligase 4CL in Zhongdou 27 (high isoflavone content variety) in the leaves were significantly higher than that in Chuxiu (low isoflavone content variety) during the R2 growing period. Meanwhile, the expression levels of chalcone synthase CHS and isoflavone synthase IFS in Zhongdou 27 were significantly higher than that in Chuxiu during the R8 growing period in the seeds. While the expression level of cytochrome P450 reductase CPR in Chuxiu was significantly higher than that in Zhongdou 27 during the R7 period in both of the leaves and seeds. Thus, these differential expression levels of related genes in different developmental stages and organs may be the main reasons to explain different seed isoflavone contents in soybean.