60份优质番茄自交系遗传多样性AFLP分析

本文以AFLP技术对60份优质番茄自交系的遗传多样性进行了分析。17对AFLP引物组合共扩增得到905条带，其中多态性条带251条，平均每对引物检测到约15个多态性位点，平均多态率为27.7%；仅用E7M4和E7M10两对引物组合就可以绘制60份番茄自交系的指纹图谱。60个自交系间的Jaccard遗传相似系数变幅为0.259-0.952，平均值为0.664。采用UPGMA方法聚类，遗传相似系数变幅为0.54-0.57时，60份番茄材料可以划分为7个类群。聚类结果与来源、果实大小、果形等性状未表现出明显的相关性。

Study on Genetic Diversity of 60 Tomato Inbred Lines with Good Quality Based on AFLP Markers

AFLP markers were used to evaluate genetic diversity among 60 tomato inbred lines with good quality. Total 905 bands were amplified using 17 AFLP primer combinations, and 251 bands of them were polymorphic bands. Average number of polymorphic loci detected by per primer combination was 15, and mean polymorphism rate was 27.7%. Unique fingerprints of 60 tomato inbred lines were obtained by a minimum of 2 AFLP primer combinations, E7M4 and E7M10. Genetic similarity coefficients of 60 inbred lines ranged from 0.259-0.952 with a mean of 0.664. 60 tomato materials can be divided into seven groups at similarity coefficients from 0.54-0.57 when UPGMA clustering was used. It was showed clustering results had no pronounced relation with phenotypic traits, such as sources, fruit size, fruit shape, and so on.