利用SSR标记构建萝卜种质资源分子身份证

为了保护我国特有的优异萝卜资源,促进资源的有效区分和合理利用,保障我国萝卜产业发展,目前需积极开展萝卜种质资源的特异性鉴定和种质识别技术研究。本研究基于SSR分子标记、信息处理和图像处理技术,筛选出22对SSR引物对75份来源和特征不同的代表性萝卜种质进行鉴定,共扩增出153条带,其中多态性条带为87条,平均多态性位点百分率为55.49%,平均每对引物可扩增出6.95条带和3.95条多态性带,有效地显示出每份萝卜种质的特异性。基于最少引物鉴定最多种质的原则,利用MATLAB程序筛选出8对SSR引物,依据8对引物的扩增数据,经过多态性谱带的有序编码转换,构建出75份萝卜种质分子身份证。结果显示利用SSR标记构建萝卜种质分子身份证进行种质资源的鉴定和保护是可行的。

Establishment of the molecular identification for radish germplasm using SSR Markers

153 bands were generated among 75 radish germplasms using 22 pairs of SSR primers which were screened out. Of them, 87 bands were polymorphic. The percentage of polymorphic alleles was 55.49%. On average, the number of alleles per SSR locus was 6.95 in total and 3.95 of polymorphic bands The genetic diversity among these radish germplasms was rich. After raw data processing and filtering by MATLAB, 75 radish germplasm could been distinguished and their molecular identities were constructed usingr 8 pairs of SSR primers. It showed that it is feasible to employ SSR markers to construct the molecular identities for elite germplasm resource and new varieties in radish.