茶陵野生稻导入系遗传分析及重要性状相关QTL鉴定

普通野生稻是栽培稻的祖先种,从野生稻中挖掘栽培稻中已丢失或削弱了的优异基因是保证水稻高产稳产的一个重要手段。本研究以茶陵野生稻染色体片段导入系群体为研究材料,使用均匀分布于12条水稻染色体上的136个多态性分子标记,对其遗传背景进行检测。平均每个导入系携带24个导入片段,导入片段平均长度为16.1 cM,导入片段覆盖野生稻基因组的87.89%。结合两个环境中的性状调查,鉴定到与6个产量性状相关的18个QTLs,其中6号染色体上与粒宽有关的QTL在两个环境中被连续检测到。除此之外,还鉴定到5个与发芽期耐冷性有关的QTLs。

Genetic Analysis of the Introgression Lines Derived from Chaling Wild Rice and Identification of Yield Related QTLs

Oryza rufipogon Griff.served as an important rice germplasm which harbors excellent yield and stress tolerance genes that have been lost or weakened in cultivated rice.Within this study,a set of introgression lines with chromosome segments derived from Chaling wild rice were used for genetic analysis and QTL identification.136 polymorphic markers evenly distributed across the 12 chromosomes were deployed for genotyping.87.89%of the Chaling wild rice genomes were detected.Averagely,24 introgression segments were found in each introgression line,and the genetic distance of each segment was accounting for about 16.1 cM.By taking use of phenotypic datasets from two environments,eighteen QTLs on six yield traits were identified.Especially,a QTL associating with grain width on chromosome 6 was found in two environments.Furthermore,five QTLs related to cold tolerance at the germination stage were detected.Taken together,these introgression lines served as elite gene resources with a potential for rice breeding.