基于SSR标记分析大豆疫霉根腐病抗源的遗传多样性

丰富的遗传多样性可为大豆育种提供宽阔的遗传基础,本研究基于35对SSR标记,对60份东北地区大豆疫霉根腐病抗性品种进行了遗传多样性分析,共检测到189个等位基因,平均每个位点等位变异数5.4个,多态性信息含量指数(PIC)为0.1550~0.8195,平均为0.6636;遗传相似系数的变异范围为0.31~0.74。利用5对高多态性SSR引物构建了60份抗性材料的指纹图谱,这5对SSR引物构建的指纹图谱可以将60份疫霉根腐病抗性材料逐一区分开。采用NTSYS2.10基于遗传距离的聚类分析,将60份抗性材料分为7个类群,其中78.33%的抗性品种(系)的遗传相似系数在0.45~0.74间,表明遗传差异相对较窄,品种间遗传多样性水平较低。聚类分析与群体遗传结构分析结果有部分重合,均反映出不同地区的抗性材料间存在一定的渗透和交流。

Genetic Diversity of Resistance to Phytophthora Root Rot Based on SSR Markers

The abundant genetic diversity provides a broad genetic basis for soybean breeding.Based on 35 pairs of SSR markers,the genetic diversity of 60 soybeans being resistant to Phytophthora root rot in Northeast China was analyzed.A total of 189 alleles were detected,with an average of 5.4 alleles per locus.The polymorphism information content index(PIC)was variable from 0.1550 to 0.8195 with an average of 0.6636.The genetic similarity coefficient varies from 0.31 to 0.74.The fingerprint map of 60 resistant varieties was constructed using 5 pairs of highly polymorphic SSR primers.The fingerprints can distinguish 60 resistant materials of Phytophthora sojae root rot one by one.Using NTSYS2.10 clustering analysis based on genetic distance,sixty resistant genotypes were divided into 7 groups,of which 78.33%of resistant varieties(lines)had a similarity coefficient between 0.45 and 0.74.That indicated a relatively narrow genetic difference among these resistance-conferring genotypes.The results of cluster analysis and population genetic structure analysis partially overlap,suggesting the existence of multiple penetration and communication events between resistant materials in different regions.