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繁殖群体量及隔离对蚕豆种质遗传完整性的影响
引用本文:刘 敏,辛 霞,张志娥,陈晓玲,张金梅,卢新雄.繁殖群体量及隔离对蚕豆种质遗传完整性的影响[J].植物遗传资源学报,2012,13(2):175-181.
作者姓名:刘 敏  辛 霞  张志娥  陈晓玲  张金梅  卢新雄
作者单位:1. 中国农业科学院作物科学研究所,北京100081;中国农业大学农学与生物技术学院,北京100193
2. 中国农业科学院作物科学研究所,北京,100081
基金项目:中国农业科学院作物科学研究所中央级公益性科研院所基本科研业务费专项,农作物种质资源保护利用专项
摘    要:为研究繁殖群体量和隔离方式对常异花授粉作物蚕豆种质繁殖更新的影响,以9份蚕豆地方种质为对象,以国家库保存的原种为对照群体,采用AFLP分子标记方法,对比了50株和20株群体量及开放无隔离群体和网棚隔离群体更新后代的遗传完整性差异。结果表明:与对照群体相比,50株和20株群体的多态性位点数、多态位点百分率、每位点有效等位基因数、香农指数、遗传多样性指数均出现不同程度下降,但下降幅度20株大于50株群体;遗传相似性和UPGMA聚类分析表明50株群体与对照群体的遗传相似性高于20株群体;网棚隔离可降低群体间串粉与花粉污染,但其遗传完整性却较开放无隔离群体低。

关 键 词:蚕豆  繁殖更新  AFLP  遗传完整性  种质保存
收稿时间:2011/5/10 0:00:00
修稿时间:2011/12/12 0:00:00

Effect of Sample Sizes and Isolation Methods on Genetic Integrity of Faba Bean(Vicia faba L. )Germplasm
liu min,and.Effect of Sample Sizes and Isolation Methods on Genetic Integrity of Faba Bean(Vicia faba L. )Germplasm[J].Journal of Plant Genetic Resources,2012,13(2):175-181.
Authors:liu min  and
Institution:China Agricutural University,,,,,
Abstract:The genetic integrity of nine faba bean landraces and their regeneration groups were studied to investigate the effects of sample sizes and isolation methods on the regeneration of faba bean (vicia faba L.) using AFLP markers. Seeds stored in the national genebank were used as CK. The results showed that: compared with those of the control group, the number of polymorphic loci, percentage of polymorphic loci, effective number of alleles per locus, Shannon index, and genetic diversity index decreased in 50 and 20 plants groups, but the decline was greater in 20 plants group; genetic similarity analysis and UPGMA cluster showed that the genetic similarity between CK and 50 plants group was higher than that between CK and 20 plants group. Cages isolation can reduce pollen contamination, but their genetic integrity was lower than that of the open pollination groups.
Keywords:Vicia faba L    Regeneration  AFLP  Genetic integrity  Germplasm conservation
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