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大豆类钙调磷酸酶B亚基GmCBL1互作候选蛋白的筛选
引用本文:李林蔚,张双喜,周永斌,裴丽丽,陈明,李连城,马有志,刘生祥,徐兆师.大豆类钙调磷酸酶B亚基GmCBL1互作候选蛋白的筛选[J].植物遗传资源学报,2015,16(2):359-363.
作者姓名:李林蔚  张双喜  周永斌  裴丽丽  陈明  李连城  马有志  刘生祥  徐兆师
作者单位:中国农业科学院作物科学研究所
基金项目:转基因生物新品种培育科技重大专项(2014ZX08002-002,2014ZX08003-004B)
摘    要:Ca2+是非生物胁迫信号转导途径中的重要信号分子,植物类钙调磷酸酶B亚基蛋白(CBL,calcineurin B-like proteins)是一类重要的钙信号受体蛋白,主要通过与其他蛋白的特异结合传递信号,使植物形成对非生物胁迫的响应。本实验室已经获得大豆Gm CBL1基因,功能鉴定显示Gm CBL1增强了转基因拟南芥对非生物胁迫的耐性。为了进一步研究Gm CBL1的作用机理,本研究构建诱饵载体p GBKT7::Gm CBL1,利用酵母双杂交技术筛选大豆Gm CBL1的互作蛋白。通过对筛选获得的106个蛋白基因测序和Blast比对分析,并根据其可能的生理功能对这些候选蛋白归类,整理得到4类蛋白:能量代谢相关蛋白、修饰蛋白、防御蛋白、钙信号转导相关蛋白。筛选得到候选蛋白的功能预测初步表明,大豆Gm CBL1参与多条信号途径,为进一步研究探索大豆CBL介导的抗逆信号转导途径奠定了基础。

关 键 词:大豆  类钙调磷酸酶B亚基  GmCBL1  酵母双杂交  互作蛋白
收稿时间:2014/9/18 0:00:00
修稿时间:2014/10/16 0:00:00

Screening of Interacting Proteins of the Calcineurin B-like Protein GmCBL1 in Soybean
Xu Zhao-Shi.Screening of Interacting Proteins of the Calcineurin B-like Protein GmCBL1 in Soybean[J].Journal of Plant Genetic Resources,2015,16(2):359-363.
Authors:Xu Zhao-Shi
Institution:LI Lin-wei;ZHANG Shuang-xi;ZHOU Yong-bin;PEI Li-li;CHEN Ming;LI Lian-cheng;MA You-zhi;LIU Sheng-xiang;XU Zhao-shi;Agricultural College of Ningxia University;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Biology and Genetic Improvement of Triticeae Crops,Ministry of Agriculture;Institute of Crop Science,Ningxia Academy of Agricultural Sciences;
Abstract:Calcium is a critical messenger in abiotic stress responses signal transduction pathways in plants. Calcineurin B-like proteins (CBLs) represent a family of plant calcium sensor protein that function in calcium signaling by interacting with their interacting protein to transduce signal, making plant response to abiotic stress. Our laboratory has identifies a putative soybean CBL gene that functions in abiotic stress tolerance tolerance in the transgenic Arabidopsis plants. In order to further explore the GmCBL1-mediated stress resistance mechanism, the mixture of bait vectors pGBKT7-GmCBL1 were constructed, pGADT7 and soybean cDNA library was introduced into yeast competent cells AH109 to screen the interaction proteins from soybean cDNA library by yeast-two hybrid system. A total of 106 candidate positive clones were sequenced, analyzed through BLAST. According to their possible physiological function , those candidate proteins were classified into one of four categories: energy metabolism proteins, modified protein, defense protein, calcium signaling transduction proteins. Function prediction of the candidate proteins suggested that GmCBL1 was possibly involved in several stress signal transduction pathways.This work provides a basis for a defined further functional dissection of soybean CBL mediated signaling system.
Keywords:soybean  calcineurin B-like proteins  GmCBL1  yeast two hybrid  interaction protein
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