利用RAPD技术鉴定海南荔枝品种光明

应用RAPD标记对6份无性系材料进行检验，20个引物对6份材料均扩增出完全相同的125条带，不具多态性，证明6份材料遗传基础高度相似。利用30个引物对光明和其他42份材料的基因组进行多态性分析，多态性位点为212个，占总数的80、83％，显示多态性较高。光明和其他荔枝品种间的相似系数均较高，与大丁香的相似系数（0．884）最高。在相似系数0．78水平上，可将供试材料分成4大类，这些类型与成熟期有一定相关，光明属于第3类。光明不具特征谱带，但可用少数引物组合将其和很多品种区别开，如利用AP09、AM16和AK17即可将光明从其他荔枝材料中鉴别出来。

Identification and Analysis of Litchi Cultivar Guangming in Hainan by RAPD Markers

Identification of six litchi putative clones with 20 arbitrary primers of 10 bases were carride out by random amplified polymorphic DNA(RAPD)markers.Each of them had the same 125 bands,which affirmed that they were high similar to type of Guangming.The genetic relationship of the 43 litchi germplasm resources were analyzed by RAPD with 30 arbitrary primers of 10 bases.212 RAPD loci were polymorphic and accounted for 80.83% of all the loci.The 43 accessions could be classified into 4 groups at the level of the similarity coefficient of 78%,and Guangming belonged to Group 3.These groups were generally with traits of maturing periods.No characteristic band was found to identify Guangming from other germplasms,but it can be identified from others by a combination of several primers such as AP09,AM16 and AK17.