| 河北省绿子叶黑豆种质资源表现型和ISSR标记遗传多样性分析 |
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| 引用本文： | 耿立格,王丽娜,张磊,宋彦峰.河北省绿子叶黑豆种质资源表现型和ISSR标记遗传多样性分析[J].植物遗传资源学报,2010,11(3):266-270. |
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| 作者姓名： | 耿立格 王丽娜 张磊 宋彦峰 |
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| 作者单位： | 1. 河北省农林科学院粮油作物研究所/河北省作物遗传育种实验室,石家庄,050031
2. 河北农业大学农学院,保定,071001 |
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| 基金项目： | 河北省科技支撑项目，河北省科技基础条件平台建设项目 |
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| 摘 要： | 为了揭示河北省绿子叶黑豆种质资源的遗传多样性,为其研究利用提供理论根据,以46份原产河北省的绿子叶黑豆种质资源为试验材料,对其基于表型性状及ISSR标记鉴定结果进行了聚类分析,结果表明:7个ISSR引物共检测出60个等位变异,平均每个位点有8.6个等位变异,变幅为5～17个;ISSR引物的多态性信息量PIC变幅为0.721～0.927,平均0.820;利用表型性状和ISSR标记数据进行品种间遗传多样性分析,遗传相似系数变化范围分别为0.07～0.53和0.43～1.00,平均为0.284和0.704,遗传相似性变幅较大,河北省不同绿子叶黑豆品种间存在着丰富的遗传多样性。聚类结果显示,类群与品种来源地有关。
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| 关 键 词： | 绿子叶黑豆 种质资源 农艺性状 ISSR 遗传多样性 |
Genetic Diversity Analysis of Green-Cotyledon Black Soybean Germplasm Resources in Hebei Province based on Agronomic Traits and ISSR Markers |
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| GENG Li-ge,WANG Li-na,ZHANG Lei,ZHANG Jing-hui,SONG Yan-feng.Genetic Diversity Analysis of Green-Cotyledon Black Soybean Germplasm Resources in Hebei Province based on Agronomic Traits and ISSR Markers[J].Journal of Plant Genetic Resources,2010,11(3):266-270. |
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| Authors: | GENG Li-ge WANG Li-na ZHANG Lei ZHANG Jing-hui SONG Yan-feng |
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| Institution: | GENG Li-ge1,WANG Li-na1,ZHANG Lei1,ZHANG Jing-hui,SONG Yan-feng 2(1Institute of Cereal , Oil Crops,Hebei Academy of Agricultural , Forestry Sciences/Crop Genetics , Breeding Laboratory of Hebei Province,Shijiazhuang 050031,2College of Agronomy,Agricultural University of Hebei,Baoding 071001) |
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| Abstract: | The genetic diversity of green-cotyledon black soybean cultivars native to Hebei province was evaluated based on ISSR and agronomic characters.The results indicated that 60 alleles of 7 ISSR markers were detected among 46 cultivars with an average of 8.57 alleles per ISSR marker,and the number of alleles per primer ranged from 5 to 17.The value of allelic polymorphism information content(PIC) ranged from 0.721 to 0.927,on the average of 0.82 per primer.The value of genetic similarity(GS) indices of 46 culti... |
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| Keywords: | ISSR Green-cotyledon black soybean Germplasm Agronomic character ISSR Genetic diversity |
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