SSR标记分析种子老化及繁殖世代对大豆“中黄18”种质遗传完整性的影响

选取大豆品种中黄18为试验材料，利用60对SSR核心引物对经过不同老化时间处理的群体及其繁殖后代群体进行遗传完整性分析。经过老化处理的群体及其繁殖子代群体与未经老化处理的对照群体相比，等位基因频率、有效等位基因数没有显著差异，结果表明经过老化处理种子及其繁殖子代群体的等位基因频率变化不大。未经老化处理、发芽率为98％的对照群体（G0-1）与其繁殖一代、繁殖二代群体相比，等位变异数、遗传多样性指数、香农指数和稀有等位基因数没有显著差异，且遗传一致度相对较高；而经过老化处理、发芽率低于85％的群体（G0-3和G0-4）及其后代繁殖群体与对照群体相比，等住变异数、遗传多样性指数、香农指数和稀有等位基因数显著或极显著降低，且遗传一致度相对较低。因此，种子老化较繁殖世代对大豆种质群体的遗传结构影响更大。

Effect of Seed Aging and Regeneration on Genetic Integrity in Soybean by SSR Markers

In this study soybean cultivar Zhonghuang 18 was used as materials,and the genetic integrity of population with different germination percentage and their descendant population was investigated by 60 core SSR primers.The results showed that there was no significant difference in the allelic frequencies and the effective number of alleles of the aged population and their descendant population compared with the control population.It indicated that the allelic frequencies of the aged population and their desce...