抗拿捕净晋谷21突变体的筛选和分子鉴定

谷子(Setaria italica(L.)P.Beauv.)是我国重要的杂粮作物,具有抗旱耐贫瘠、水分利用率高、适应性广、营养丰富等特点,但谷子田间除草一直制约着谷子的集约化栽培和有机旱作产业规模化的推广与发展。为快速选育抗除草剂谷子品种,本试验以晋谷21突变体M2群体为试验材料,通过喷施0.33%拿捕净除草剂对晋谷21突变群体进行初次筛选和再次筛选,进而快速获得抗拿捕净除草剂突变体植株并对其进行分子鉴定。结果显示,在大田苗期喷施0.33%拿捕净除草剂的初次筛选中,筛选出抗拿捕净除草剂的晋谷21突变体共39个株系;对这39个突变体株系再次喷施0.33%拿捕净除草剂,筛选获得9个有抗拿捕净除草剂特性的株系。对筛选获得的抗拿捕净突变体株系的其中3个株系共15个植株进行分子鉴定,发现有4个突变体植株的ACCase基因编码的第1780个氨基酸-异亮氨酸突变为亮氨酸。该结果可为今后利用分子生物学手段改良名优谷子品种晋谷21、加速抗除草剂谷子品种的选育提供理论和材料基础。

Screening and Molecular Identification of Mutants of Sethoxydim-resistant Foxtail Millet Jingu 21

Foxtail millet(Setaria italica(L.)P.Beauv.)is a significant minor crop in China,having the characteristics of drought resistance and infertile soil tolerance,high water using efficiency,wide adaptability and rich nutritious grains.However,the weeding problem has been restricting intensive cultivation and promotion and development of organic dry farming industry of foxtail millet.In order to quickly select herbicide-resistant foxtail millet varieties,the M2 population of mutants of foxtail millet Jingu 21 was used as the test materials.The initial selection and re-screening of the Jingu 21 mutants population were carried out by spraying 0.33%sethoxydim herbicide in order to quickly obtain mutant plants and identify their molecular characteristics.The results showed that 39 mutant plants resistant to sethoxydim were preliminarily selected in the initial screening of 0.33%sethoxydim sprayed at the seedling stage in the field.From these 39 mutant plants,9 mutants were identified after respraying 0.33%sethoxydim.A total of 15 plants of 3 mutant strains were molecularly identified,and it was found that the 1780 th amino acid encoded by the ACCase gene was mutated from isoleucine to leucine in 4 mutant plants,while no change was found in other plants.The results would provide research materials for improvement of the elite millet variety Jingu 21 and accelerate breeding of herbicide-resistant millet varieties.