滇蔗茅杂交F_1双抗SCSMV和SrMV鉴定与评价

以我国蔗区甘蔗花叶病的2种主要病原甘蔗条纹花叶病毒分离物(SCSMV-JP1,Gen Bank登录号JF488064)和高粱花叶病毒分离物(Sr MV-HH,Gen Bank登录号DQ530434)为接种毒源,采用人工切茎接种和RT-PCR检测相结合的方法,于2015-2016年2次对由热带种路打士与滇蔗茅云滇95-19杂交获得的41份滇蔗茅杂交F_1及亲本进行了双抗SCSMV和Sr MV鉴定与评价。结果表明,41份滇蔗茅杂交F_1及亲本中,对SCSMV表现1级高抗到3级中抗的有23份,占53.49%,4级感病到5级高感的有20份,占46.51%;对Sr MV表现1级高抗到3级中抗的有31份,占72.09%,4级感病到5级高感的有12份,占27.91%。综合分析结果显示,10份滇蔗茅杂交F_1对SCSMV和Sr MV均表现1~2级抗病,占23.26%,其中云09-604、云09-607、云09-619、云09-633、云09-656、云滇95-19等6份滇蔗茅杂交F_1对2种病毒均表现为1级高抗,占13.95%。研究结果明确了41份滇蔗茅杂交F_1及亲本对甘蔗花叶病2种主要致病病原的抗性,筛选出10份双抗SCSMV和Sr MV的滇蔗茅杂交F_1,为深入开展抗甘蔗花叶病育种提供了优良抗源种质和参考依据。

Identification and evaluation of elite sugarcane innovation germplasm resistant to SCSMV and SrMV

In 2015 and 2016,the two main pathogenic Sugarcane streak mosaic virus (SCSMV-JP1,GenBank accession number JF488064)and Sorghum mosaic virus (SrMV-HH,GenBank accession number DQ530434)were used as inoculums,and the double resistance to SCSMV and SrMV was identified and evaluated in 41 F1 hybrids of Saccharum officinarum L."Ludashi" × Erianthus rockii Keng "Yundian95-19" and parents by dropping inoculation on stemsection and RT-PCR detection.The results showed that among the 41 Erianthus rockii Keng F1 hybrids and parents,23 (53.49％) were highly (Grade 1) to moderately resistant (Grade 3),and 20 (46.51％) were susceptible (Grade 4) to highly susceptible (Grade 5) to SCSMV;31 (72.09％) were highly (Grade 1) to moderately resistant (Grade 3),and 12 (27.91％) were susceptible (Grade 4) to highly susceptible (Grade 5) to SrMV.Ten Erianthus rockii Keng F1 hybrids (23.26％)were highly resistant (Grade 1)to resistant (Grade 2)to SCSMV and SrMV,that is,Yun09-603,Yun09604,Yun09-607,Yun09-608,Yun09-619,Yun09-622,Yun09-633,Yun09-635,Yun09-656 and Yundian95-19.Among these,six Erianthus rockii Keng F1 hybrids (Yun09-604,Yun09-607,Yun09-619,Yun09-633,Yun09-656,Yundian95-19) were highly resistant (Grade 1)to SCSMV and SrMV,accounting for 13.95％ of the total germplasm.The results in the present study have determined the resistance of 41 Erianthus rockii Keng F1 hybrids and parents to the two main pathogens of mosaic disease,screened out 10 Erianthus rockii Keng F1 hybrids resistant to SCSMV and SrMV,which provides scientific basis and valuable resistance resource for breeding of sugarcane cultivars against mosaic disease.