小麦一个新BBI型蛋白酶抑制剂基因TaUES的克隆及初步分析

根据小麦基因表达芯片结果,以山融3号小麦叶片为试验材料,克隆获得在盐胁迫处理24 h时表达显著提高的基因TaUES(up-regulated expression under saline-stress in wheat,ID:KC408382)。序列分析显示,TaUES编码一个富含半胱氨酸的97个氨基酸的多肽,该多肽含有1个BowB结构域和10个保守的半胱氨酸残基;与小麦或其他物种BBI型蛋白酶抑制剂氨基酸序列具有较高的同源性。推测TaUES是小麦中一种新的BBI型蛋白酶抑制剂基因。基因表达分析表明,TaUES基因参与盐和干旱胁迫应答。异源过表达转基因功能初步分析表明,过表达TaUES转基因烟草后代株系耐盐性提高。

Cloning and preliminary analysis of a new BBI-type protease inhibitor gene TaUES from wheat

A gene, named as TaUES (up-regulated expression under saline-stress in wheat, ID: KC408382) expressing significantly after NaCl treatment for 24hrs, was cloned from the leaves of wheat variety Shanrong No.3 based on the results of wheat gene chips, and it encoded a cysteine-rich polypeptide with 97 amino acids, containing a BowB domain and 10 conserved cysteine residues. The analysis of amino acid sequence indicated that this polypeptide showed high homologue to those of BBI-type protease inhibitors from wheat or other species, implying that TaUES may be a gene encoding a new wheat BBI-type protease inhibitor. TaUES expression suggested that it was involved in salt and drought stress response. Preliminary analysis of ectopic gene expression showed that over-expression of TaUES in transgenic tobacco lines resulted in their high tolerance to salt stress.