鉴定烟草种质资源SSR核心引物筛选和验证

核心引物对遗传多样性分析、种质资源鉴定、品种纯度和真实性检测、指纹图谱构建等研究具有重要价值。本研究利用均匀分布于烟草24条染色体的278对SSR引物，对20份亲缘关系相对较远的烟草材料进行初步筛选，筛选出32对引物。随后再加上10份亲缘关系较近的材料（共30份）对引物进行复筛，最终确定14对为核心引物。将14对引物对39份烟草材料进行进行系谱分析、品种遗传多样性分析和农艺性状分析聚类，结果表明该套SSR核心引物适用于烟草种质资源鉴定和遗传多样性分析。

Screening Of SSR Core Primer Pairs for Identification Tobacco

Core primers are important for genetic diversity analysis, germplasm identification, purity and genuineness test, and fingerprint establishment. Currently, we have screened 20 tobacco germplasm stocks with distant relationships by using of 278 SSR primer pairs, which evenly distribute on 24 chromosomes, through the strategy of distant relationships, and among which 32 primer pairs were selected out. And then, another 10 germplasm stocks with similar relationship were used to screen the 32 primer pairs, and 14 SSR core primer pairs were obtained. These 14 SSR core primer pairs were conducted on pedigree research, genetic diversity analysis and agronomic traits clustering study for 39 germplasm stocks. The result showed these core primer pairs could be applied to germplasm identification and genetic diversity study in tobacco.