中国新疆野苹果天然群体遗传多样性SSR分析

摘要：本研究以新疆巩留、新源、霍城、托里4个居群下的12个新疆野苹果群体为材料，应用涉及12个连锁群的17对SSR引物进行了群体微卫星位点等位基因和基因型差异分析，从地理居群、海拔高度、引物类型角度对新疆野苹果天然群体遗传多样性和遗传结构进行了 探索。结果表明：17对SSR引物在新疆野苹果中种内多态位点百分率达100%；XY1、HC2群体的基因多样性较高。聚类结果显示，同一居群下的群体分在相同的组中；巩留居群与新源居群的遗传关系最近，霍城居群次之，托里居群最远。不同地区间遗传多样性，霍城居群最高，托里居群最低。海拔高度对群体遗传结构影响较小，除了观测杂合度与海拔存在弱正相关外，大多数遗传参数与海拔无相关性。新疆野苹果群体内变异大于群体间，群体间分化很小，基因流较高。

Stduy on Genetic Diversity of Natural Population in Malus sieversii with Microsatellite

Abstract: In the study,we used 12 wild apples groups of Xinjiang which undered the four populations of Xinjiang Gongliu,Xinyuan,Huocheng,Tuoli as materials,involving in group microsatellite allele and genotype differences analysis of 12 linkage groups with 17 pairs of SSR primers,exploring genetic diversity and genetic structure of natural populations of Xinjiang wild apples from geographic populations,the altitude and the type of primer perspective.The results showed that the percentage of intraspecific polymorphic loci in Xinjiang wild apples with 17 pairs of SSR primers was up to 100%;the genetic diversity of group XY1 and HC2 were higher.Clustering results showed that the groups undered the same population were divided in the same group;The genetic relationships of the Gongliu population and the Xinyuan population were nearest,the Huocheng population was followed,the Tuoli population was the farthest.The Huocheng population was the highest,The Tuoli population was the lowest on genetic diversity in the different regions.The impact on the population genetic structure of altitude was smaller,excepting the observed heterozygosity and altitude exist weak positive correlation,most genetic parameters had no correlation with altitude.The variation within Xinjiang wild apples populations was greater than among populations,the differentiation among populations was small,the gene flow was higher.