| 红根甘肃桃根系MYB基因片段的克隆及序列分析 |
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| 引用本文： | 魏潇,曹珂,王力荣,朱更瑞,方伟超,陈昌文.红根甘肃桃根系MYB基因片段的克隆及序列分析[J].植物遗传资源学报,2011,12(3):455-459. |
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| 作者姓名： | 魏潇 曹珂 王力荣 朱更瑞 方伟超 陈昌文 |
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| 作者单位： | 中国农业科学院郑州果树研究所,郑州,450009 |
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| 基金项目： | 国家自然科学基金项目，国家"863"计划项目 |
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| 摘 要： | 为了明确MYB转录因子在红根甘肃桃抗性分子机制中的作用.通过RT-PCR从红根甘肃桃根系cDNA中克隆了14个MYB基因片段,序列分析表明这些片段与苹果、葡萄、拟南芥、番茄等植物的MYB转录因子高度同源;系统进化分析显示红根甘肃桃的11个PkMYB分别与拟南芥、番茄、杨树、柿等植物中已知功能的MYB转录因子聚在不同的亚类,据此推测它们可能有相似的功能.试验结果为进一步研究MYB基因在红根甘肃桃抗性机制中的作用奠定了基础.
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| 关 键 词： | 红根甘肃桃 根系 MYB基因 克隆 序列分析 |
Cloning and Characterization of MYB Genes from Root System of Honggengansutao Peach(Prunus kansuensis L.) |
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| WEI Xiao,CAO Ke,WANG Li-rong,ZHU Geng-rui,FANG Wei-chao,CHEN Chang-wen.Cloning and Characterization of MYB Genes from Root System of Honggengansutao Peach(Prunus kansuensis L.)[J].Journal of Plant Genetic Resources,2011,12(3):455-459. |
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| Authors: | WEI Xiao CAO Ke WANG Li-rong ZHU Geng-rui FANG Wei-chao CHEN Chang-wen |
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| Institution: | WEI Xiao,CAO Ke,WANG Li-rong,ZHU Geng-rui,FANG Wei-chao,CHEN Chang-wen(Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009) |
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| Abstract: | In order to identify the molecular resistance mechanism of Honggengansutao peach(Prunus kansuensis L.),14 fragments in MYB genes family were isolated from cDNA in root system of Honggengansutao peach by RT-PCR.By comparison,we found that their inferred amino acid sequences have high identity with their counterparts from apple,grape,Arabidopsis,tomato and other plants in GenBank.The result of phylogenetic analysis showed that 11 PkMYBs are clustered with known functions MYBs from Arabidopsis,tomato,poplar an... |
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| Keywords: | Honggengansutao peach(Prunus kansuensis L ) Root system MYB genes Cloning Sequence analysis |
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