甜瓜属种间杂交线粒体DNA的遗传分析

为了研究甜瓜属种间杂交线粒体DNA的遗传规律，对甜瓜属人工杂交合成的异源四倍体新种(Cucumis hytivus Chen and Kirkbride.)的S5后代及其杂交母本甜瓜属野生种（Cucumis hystrix Chakr.）和父本栽培种黄瓜‘北京截头’(Cucumis sativus L.)线粒体中apocytochrome b (cob), NADH dehydrogenase subunit 1 (nad 1), NADH dehydrogenase subunit 7(nad 7) 基因序列片断进行了测序与分析。结果显示3个种长度为909 bp cob, 943 bp nad 1和880 bp nad 7基因片断序列中分别存在着相应的1、7和17个多态性核苷酸位点，其中新种与父本‘北京截头’相同而与母本野生种不同的多态性碱基位点分别有1、6和14个，nad 1和nad 7中分别只有1和3个多态性位点与双亲都不相同。该结果表明甜瓜属hystrix譻ativus种间杂交后代线粒体DNA多态性碱基位点主要来源于父本而不是母本，线粒体DNA主要表现为父系遗传。

Inheritance Analysis of Mitochondrial(mt)DNA in the Interspecific Crossing of Genus Cucumis

To investigate the inheritance of the mitochondrial(mt)DNA in the interspecific crossing of Genus Cucumis,fragments of the cob,nad 1,nad 7 mt gene were amplified and sequenced from S5 progenies of allotetraploid species(Cucumis hytivus Chen and Kirkbride.),cultivated cucumber 'BeijingJietou'(C.sativus L.) the paternal parents and the wild Cucumis species(C.hystrix Chakr.) the maternal parents,respectively.One,seven and seventeen polymorphic loci's in the corresponding 909 bp cob,943 bp nad 1 and 880 bp nad ...