甘蓝型特高含油量油菜种质资源及主栽品种指纹图谱构建

本研究选择特高含油量资源7份，与中国各油菜主产区具有代表性的主栽品种16份，利用SSR多引物组合法开展指纹图谱构建研究。选择多态丰富、图谱清晰稳定且来自不同连锁群的引物28对，对所有材料进行指纹图谱分析，共获SSR指纹条带302条，其中多态性条带为279条，每引物所获条带6-16条，平均10.79条，平均多态率92. 38%，通过指纹图谱将所有材料有效地区别开来。用非加权类平均法（UPGAM）聚类分析显示：高油材料之间以及高油材料与主栽品种之间遗传距离均有较大差异，在遗传距离0.171处可将23份材料分成9个类群，其中7份高油材料分处4个类群，遗传距离差异显著；而其他8份主栽品种被分别聚类在另外5个类群中；所有材料间皆具有丰富的遗传多样性，其中高油材料与主栽品种间遗传差异更大。

Fingerprinting Construction of Brassica napus germplasm with super-high oil content and main rapeseed cultivars

In this paper, we selected 7 rapeseed germplasms with super-high oil content and 16 main rapeseed cultivars from the different planted regions to construct their fingerprinting by SSR molecular markers. Using 28 SSR primer pairs from the different linkage groups, we totally got 302 SSR bands for the all materials, 279 of which are polymorphism bands, each primer amplified 6-16 bands with 10.79 bands on average, the average of polymorphism rate reach 92.38%. By this way, we could distinguish all the materials better. UPGAM analyzing revealed that there were the large difference among the high oil content germplasms and between the high oil content germplasms and the main cultivars. At genetic distance of 0.171, the 23 B. napus could be divided into nine groups, in which the super- high oil content germplasms were divided into four groups, and 8 main cultivars were divided into five groups. These results showed that all materials had the most abundant genetic diversity, especially there were large genetic differences between the super-high oil germplasms and the main rapeseed cultivars.