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PEG胁迫条件下41份陆地棉种质资源萌发特性研究及其抗旱性综合评价
引用本文:王俊娟.PEG胁迫条件下41份陆地棉种质资源萌发特性研究及其抗旱性综合评价[J].植物遗传资源学报,2011,12(6):840-846.
作者姓名:王俊娟
作者单位:中国农业科学棉花研究所/农业部棉花遗传改良重点实验室,河南安阳,455000
基金项目:中央级公益性科研院所基本科研业务专项(SJA1001),国家转基因重大专项 (2008ZX08005-004),“十一五” 国家科技支撑计划(2006BAD13B04-1),国家高技术研究发展计划(863计划) (2009AA101104)
摘    要:摘要:干旱是一种广泛存在且严重影响作物及棉花的产量的一种自然灾害。本实验采用15%PEG6000竖直滤纸法对41份陆地棉种子进行萌发期抗旱性进行研究,分别测定在胁迫条件下及清水对照条件下的发芽势、发芽率、3天芽长、3天芽重、7天下胚轴长、7天胚根长、胚根长/胚芽长、芽长生长率等8个指标,结果表明,这8个指标在PEG6000胁迫条件下,均比对照降低,除3天芽重外,41个材料的变异系数均大大增加,说明陆地棉种子萌发受15%PEG6000的抑制,且材料间受抑制程度差异较大。同时计算出胁迫条件下与对照条件的相对值,通过对这8个指标的相对值进行方差分析和主成分分析,筛选出相对发芽势、相对发芽率、相对7天下胚轴长、相对7天胚根长、相对胚根长/胚芽长、芽长生长率等7个指标与陆地棉萌发期抗旱性有关。利用这7个指标对41个陆地棉材料进行隶属函数分析和相关性分析,并以平均隶属函数值代表抗旱性强弱,平均隶属函数值越大,抗旱性越强,结果表明,相对7天胚根长与平均隶属函数值存在着极显著的正相关(r=0.839**),同时筛选出陆地棉萌发期抗旱性较强的材料有7份:冀668、鲁棉研21、9409选系、DP99B、创棉22、sGK中980、邯177,抗旱性中等的材料有10份:中23A-12、中23A抗棉F12、中棉所35、冀1286、GKZ19F6、双豫97-2067、光籽2、耐高温-2、中S9612、光籽1。 关键词:萌发期;棉花抗旱性;主成份分析;隶属函数法

关 键 词:萌发期  棉花抗旱性  主成份分析  隶属函数法
收稿时间:1/20/2011 3:38:00 PM
修稿时间:2011/4/11 0:00:00

Germination Characteristics and Comprehensive Evaluation of Drought Resistance of 41 Accessions of cotton germplasm at Seed Germination Stage under PEG-6000 Stress
wangjunjuan.Germination Characteristics and Comprehensive Evaluation of Drought Resistance of 41 Accessions of cotton germplasm at Seed Germination Stage under PEG-6000 Stress[J].Journal of Plant Genetic Resources,2011,12(6):840-846.
Authors:wangjunjuan
Institution:Chinese Cotton Research Institute of CAAS
Abstract:Abstract: Drought is a serious, worldwide problem for crop production and also affects yields of cotton. This paper conducted on different drought resistance of 41 cotton germplasm at germination stage by the experiment of 15%PEG6000 vertically rolled paper tests.We determined 8 indentification indices such as germination energy, germination rate, seedling length of 3 days, total seedling fresh biomass of 3 days, hypocotyl length of 7 days, radicle length of 7 days, radicle length of 7 days/Hypocotyl length of 7 days, growth rate of sprout length. The results showed that the 8 indices were lower under 15%PEG6000 than water controlled condition and the coefficients of variation became bigger except for total seedling fresh biomass of 3 days indicating that cotton germination was inhibited by 15%PEG6000 and the different varieties(lines) were evidently different. At the same time the relative values of T/CK were calculated. We screened 7 indentification indices such as relative germination energy, relative germination rate, relative seedling length of 3 days, relative hypocotyl length of 7 days, relative radicle length of 7 days, relative radicle length of 7 days/Hypocotyl length of 7 days, relative growth rate of seedling length by the way of variance analysis and principal component analysis. The results showed that this 7 indices had some connection with germination drought resistance. The subordinate function values of cotton germplasm under PEG6000 drought treatment and the correlation coefficient were analyzed by the 7 indices. The average subordinate function values stood for ability of germination drought resistance. With the increase of the average subordinate function values the cotton germination drought resistance ability was increasing. The results showed that relative radicle length of 7 days was significantly related to the average subordinate function values(r=0.839**). There were 7 accessions of cotton germplasm with high drought resistance ability at germination stage such as Ji668,Lumianyan21, 9409xuanxi,DP99B,Chuangmina22, sGKZhong980,Han177 and 10 accessions of cotton germplasm with medium drought resistance ability such as Zhong23A-12,Zhong23AkanmianF12,CRI35,Ji1286, GKZ19F6,Shuangyu97-2067,Guangzi2,Naigaowen-2,ZhongS9612, Guangzi1.
Keywords:Germination stage  Cotton Drought resistance  Principal component analysis  Subordination function method
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