不同大豆基因型对农杆菌敏感性研究及优异种质筛选

大豆遗传转化一直是植物基因工程领域的难点之一,受体品种对农杆菌的敏感程度以及不同农杆菌菌株对靶组织的侵染能力是影响转化效率的主要因素。本研究利用GUS组织瞬时表达技术,比较了4个农杆菌菌株对靶组织子叶节的侵染能力差异,同时利用筛选到的侵染能力最强的菌株评估了33个不同受体基因型对农杆菌的敏感性。结果表明,超毒农杆菌菌株Ag10对靶组织的侵染能力最强,优于其他3个菌株;地方与野生大豆种质在农杆菌侵染后GUS的平均瞬时表达效率显著高于选育品种。此外,通过鉴定筛选出6个对农杆菌敏感性较高的受体材料,其对农杆菌的敏感性优于前人报道的敏感材料Peking,为大豆高效遗传转化体系的建立和新型转化受体的开发提供了参考。

Assessment of Agrobacterium sensitivity for different soybean genotypes and excellent germplasm screening

Soybean is a widely planted transgenic crop worldwide. However, it is still one of the most recalcitrant crops for genetic transformation due to the genotype dependence and the infection ability of Agrobacterium. In this study, the GUS transient expression analysis was used for evaluating the Agrobacterium sensitivity for different soybean genotypes and four Agrobacterium strains. The highest transient expression efficiency of two soybean genotypes were acquired when the explants were infected by Ag10 among different stains. Meanwhile, Agrobacterium sensitivity of 33 soybean genotypes including modern cultivars, landrace and wild soybeans was assessed and six sensitivity genotypes were identified when compared with the sensitivity control Peking. The results also showed that the Agrobacterium sensitivity for landrace or wild soybean was significant higher than modern cultivars. These results are important to construct or improve the high-efficiency soybean genetic transformation system and to develop new receptors for high efficiency genetic transformation.