不同发育时期小麦粒重性状QTL的动态分析

利用6044×01-35构建的重组自交系(RIL)群体为试验材料,对小麦粒重性状进行发育动态QTL分析。结果表明,在小麦花后子粒灌浆的7个不同时期,两个试验点共检测到16个与粒重性状相关的QTL。其中开花后20d检测到的单穗粒重QTL位于2A染色体上,解释率达12%,遗传效应超过10;两环境下控制千粒重QTL在7个时期均被检测到。花后的各个时期均能在Xgwm448-Xgpw7399标记区间定位到千粒重QTL。其中花后10d检测到1个千粒重QTL,位于2A染色体的Xgwm448-Xgpw7399标记区间,解释较大的表型变异,达到18%。Qtl8、Qtl13和Qtl14均定位在Xgwm448-Xgpw7399标记区间的同一位置,共同解释11%的表型变异。花后20d和花后25d均检测到1个QTL,位于2A染色体的Xgwm372-Xgwm95标记区间的不同位点,均能解释4%的表型变异。花后40d检测到1个QTL,位于1D染色体的Xwmc93-Xgpw2224标记区间,解释1%的表型变异。从连锁群的位置上看,控制千粒重的QTL主要集中在2A染色体的Xgwm448-Xgpw7399标记区间,这是一个控制千粒重QTL的富集区域,以期进行精细定位和图位克隆。

Dynamic QTL Analysis of Kernel Weight in Wheat at Different Developmental Stages

Abstract: Kernel weught is a key factor influencing wheat processing quality ,which is determined by quantitative trait loci(QTL). In the present paper, recombinant inbred lines(RIL) population was used derived from 6044?1-35. The results showed that sixteen QTLs of kernel weight were detected in different seed filling periods totally. One QTL of kernel weight per spike detected in 10 days after flowering was an important one, which was on chromosome 2A and actcounted for 12%, and its genetic effect is more than 10. QTLs of thousand kernel weight were detected in the seven periods. One QTL of thousand kernel was detected 10 days after flowering which was on chromosome 2A, the interval from Xgwm448 to Xgpw7399 and explained 18% of phenotypic variance. One QTL was detected 15 days after flowering which was on chromosome2A, the interval from Xgwm448 to Xgpw7399 and explained 5% of phenotypic variance. Two QTLs of thousand kernel was detected 20 days after flowering which was on chromosome 2A, the interval from Xgwm448 to Xgpw7399 and Xgwm372 to Xgwm95 explained 5% and 4% of phenotypic variance. Three QTLs of thousand kernel was detected 25 days after flowering which was on chromosome 4A, the interval from Xgpw2228 to Xwmc501, and chromosome 2A, Xgwm372 to Xgwm95 and Xgwm448 to Xgpw7399, explained 2%, 4% and 8% of phenotypic variance. One QTL of thousand kernel was detected 30 days after flowering which was on chromosome 2A, the interval from Xgwm448 to Xgpw7399 and explained 3% of phenotypic variance. One QTL of thousand kernel was detected 35 days after flowering which was on chromosome 2A, the interval from Xgwm448 to Xgpw7399 and explained 3% of phenotypic variance. Two QTLs of thousand kernel was detected 40 days after flowering which was on chromosome 1D, the interval from Xwmc93 to Xgpw2224 and chromosome 2A, the interval from Xgwm448 to Xgpw7399 explained 1% and 5% of phenotypic variance. QTLs of thousand kernel weight mostly located in linkage group 2A, the interval from Xgwm448 to Xgpw7399, which was a rich domain on QTLs.