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SSR标记对不同黄籽甘蓝型油菜亲本材料的遗传分析
引用本文:刘晓兰,曲存民,谢景梅,卢坤,王敏,王瑞,徐新福,谌利,李加纳.SSR标记对不同黄籽甘蓝型油菜亲本材料的遗传分析[J].植物遗传资源学报,2012,13(4):632-638.
作者姓名:刘晓兰  曲存民  谢景梅  卢坤  王敏  王瑞  徐新福  谌利  李加纳
作者单位:重庆市北碚区西南大学农学与生物科技学院,西南大学农学与生物科技学院,西南大学农学与生物科技学院,西南大学农学与生物科技学院,西南大学农学与生物科技学院,西南大学农学与生物科技学院,西南大学农学与生物科技学院,西南大学农学与生物科技学院,西南大学农学与生物科技学院
基金项目:国家自然科学基金(31071450);农业部油菜现代产业技术体系 (CARS-13);重庆市重点项目(CSTC,2010AA1014);西南大学研究生创新基金(ky2009007);西南大学博士基金( SWU110015);国家高技术研究发展计划(863计划)
摘    要:杂交育种中,亲本选配是育种成败的关键。本研究以重庆市油菜工程技术研究中心提供的180份甘蓝型黄子油菜亲本种质为材料,应用分布于不同连锁群的60对SSR标记进行了分析,共检测出308个标记位点,每对引物在不同亲本材料之间的等位基因数在1~11个之间,平均位点为5.1个。其中多态性位点207个,多态率达67.2%。对SSR扩增结果进行UPGMA分析,在遗传距离0.566处,180个品种(系)分为3个类群,聚类结果与种质来源比较一致,本研究为甘蓝型油菜黄子杂交育种和优势组合的选配提供了理论依据。

关 键 词:甘蓝型油菜  亲本骨干系  SSR标记  核心种质  遗传多样性
收稿时间:2011/9/29 0:00:00
修稿时间:1/10/2012 7:54:01 PM

Genetic diversity of yellow-seeded parental lines of Brassica napus L by using SSR markers
Liu Xiaolan,and Li Jiana.Genetic diversity of yellow-seeded parental lines of Brassica napus L by using SSR markers[J].Journal of Plant Genetic Resources,2012,13(4):632-638.
Authors:Liu Xiaolan  and Li Jiana
Institution:1,2(1College of Agronomy and Biotechnology,Southwest University,Chongqing 400716; 2Engineering Research Center of South Upland,Agriculture of Ministry of Education,Chongqing 400716)
Abstract:To select suit parents was the key work of hybridization in Brassica napus L. The genetic diversity of 180 parental germplasm of yellow-seeded B. napus provided by the Chongqing Rapeseed Engineering Research Center of Southwest University were analyzed. 308 alleles were detected via 60 SSR primer pairs which were selected from the different linked groups in Brasicca napus, , among them 207 polymorphic bands were obtained, accounting for 67.2% of the total bands. The UPGMA cluster analysis based on SSR data showed that 180 parental lines could be classified into three groups at the threshold 0.566 of genetic distance, which agrees with the pedigree analysis. 20 samples were chosen as core collection by the pedigree, genetic distance and five quality traits of germplasm characteristics. The results showed that the SSR markers could be explained the genetic diversity B. napus accessions appropriately. It was an effective method to develop a core collection of the crop by SSR markers and germplasm characteristics. It could also provide the theoretical basis for the hybridization and breeding of yellow-seeded Brassica napus.
Keywords:Brassica napus L    Parental lines  SSR marker  Core collection  Genetic diversity  
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