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基于SRAP标记的薏苡种质资源遗传多样性及DNA指纹图谱构建
引用本文:夏法刚,黄金星,季彪俊,詹福杨,谢萍萍,邓邦柱,林宏,郑金贵.基于SRAP标记的薏苡种质资源遗传多样性及DNA指纹图谱构建[J].植物遗传资源学报,2017,18(3):413-420.
作者姓名:夏法刚  黄金星  季彪俊  詹福杨  谢萍萍  邓邦柱  林宏  郑金贵
作者单位:1. 福建农林大学作物科学学院,福州,350002;2. 浦城县农业科学研究所,浦城,353400
基金项目:青年骨干教师研修项目(201507870009),福建省科技厅重点项目(2011N0004),福建省教育厅项目(JA10111),福建省自然科学基金指导性科技计划项目(2012D087)
摘    要:利用SRAP分子标记对从各主要产地收集到的90份薏苡种质进行遗传多样性分析,其中68份收集于福建省,6份来自中国台湾,16份来自浙江、辽宁、山东、河南、云南、江苏、湖南、广东、上海等省(市)。结果表明,从88对SRAP引物组合中筛选出26对引物进行SRAP扩增,共扩增出185条带,其中具有多态性的有157,占总数的84.86%,表明90份薏苡种质表现出丰富的遗传多样性。基于SRAP标记利用系统聚类法将90份薏苡种质资源分为4大类,与形态性状分类结果有一定的相似性;利用16对SRAP引物构建了73份薏苡种质资源的DNA指纹图谱,为薏苡遗传研究、品种选育与资源保护提供了依据。

关 键 词:薏苡  种质资源  SRAP标记  DNA指纹图谱
收稿时间:2016/6/11 0:00:00
修稿时间:2016/12/28 0:00:00

Genetic Diversity Analysis and DNA Fingerprint Construction of Coix lacryma-jobi Germplasm Resources by SRAP Marker
XIA Fa-gang,HUANG Jin-xing,JI Biao-jun,ZHAN Fu-yang,XIE Ping-ping,DENG Bang-zhu,LIN Hong,ZHENG Jin-gui.Genetic Diversity Analysis and DNA Fingerprint Construction of Coix lacryma-jobi Germplasm Resources by SRAP Marker[J].Journal of Plant Genetic Resources,2017,18(3):413-420.
Authors:XIA Fa-gang  HUANG Jin-xing  JI Biao-jun  ZHAN Fu-yang  XIE Ping-ping  DENG Bang-zhu  LIN Hong  ZHENG Jin-gui
Institution:Fujian Agriculture and Forestry University
Abstract:Genetic diversity of 90 Coix lacryma-jobi germplasm resources collected from the main places of production were analyzed by Sequence-related amplified polymorphism (SRAP) marker, 68 of which were collected from Fujian, 6 from Taiwan, and another 16 from Zhejiang, Liaoning, Shandong, Henan, Yunnan, Jiangsu, Hunan, Guangdong, Shanghai etc. It shows that 26 pairs of primers were selected from 88 pairs of SRAP primers. 26 pairs of SRAP primers produced 185 amplified fragments, of which 157 were polymorphic, accounting for 84.67% of the total. It shows that there is distinct genetic diversity among 90 Coix lacryma-jobi germplasm resources. 90 Coix lacryma-jobi germplasm resources are classified into four categories basing on the UPGMA cluster analysis, which is similar to the result by morphological markers. A DNA fingerprint with 73 Coix lacryma-jobi was constructed by 16 pairs of SRAP primers. There are of significance for genetic research, variety breeding and resource protection of Coix lacryma-jobi.
Keywords:Coix lacryma-jobi  Germplasm resources  SRAP marker  DNA fingerprint
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