| 九台晚李PGIP基因的克隆及生物信息学分析 |
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| 引用本文： | 郭庆勋,张春雨,王晶莹,周连霞,王彦涛.九台晚李PGIP基因的克隆及生物信息学分析[J].植物遗传资源学报,2010,11(5):650-653. |
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| 作者姓名： | 郭庆勋 张春雨 王晶莹 周连霞 王彦涛 |
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| 作者单位： | 吉林大学植物科学学院,长春,130062 |
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| 基金项目： | 吉林大学农学部博士启动基金项目(430505010203); 长春市科技局国际合作计划项目(2D5070046202) |
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| 摘 要： | 以九台晚李叶片基因组为模板,PGIP基因保守序列设计引物,PCR扩增到1条全长1192bp的目的片段(GenBank登录号:GU068978)。该基因包含有1个完整的开放阅读框,由2个外显子和1个内含子构成,外显子总长990bp,编码330个氨基酸,其编码的氨基酸序列中含有一段典型的亮氨酸重复序列。序列分析表明:该基因与中国李、杏、桃、马哈利樱桃、梅等李属植物的PGIP基因序列一致度达95%~99%。系统进化分析显示出属内亲缘关系较近、属间亲缘关系较远的特点。该序列为植物分子抗病育种提供了1条新的基因资源。
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| 关 键 词： | 九台晚李 PGIP基因 基因克隆 生物信息学 |
Cloning and Bioinformatic Analysis of PGIP Gene from Jiutaiwanli |
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| GUO Qing-xun,ZHANG Chun-yu,WANG Jing-ying,ZHOU Lan-xia,WANG Yan-tao.Cloning and Bioinformatic Analysis of PGIP Gene from Jiutaiwanli[J].Journal of Plant Genetic Resources,2010,11(5):650-653. |
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| Authors: | GUO Qing-xun ZHANG Chun-yu WANG Jing-ying ZHOU Lan-xia WANG Yan-tao |
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| Institution: | GUO Qing-xun,ZHANG Chun-yu,WANG Jing-ying,ZHOU Lan-xia,WANG Yan-tao(College of Plant Science,Jilin University,Changchun,130062) |
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| Abstract: | A target fragment with a full length of 1192 bp(GenBank accession:GU068978) was amplified with genomic DNA of Prunus salicina L.cv.Jiutaiwanli leaves as the templates and the conservative sequences of PGIP gene as the primers.This sequence had a full open reading frame encoding the polygalacturonase-inhibiting protein,and eontained two exons interrupted by one intron.The total exons were comprised by 990bp of deoxynucleotide encoding 330 amino acid.A conserved leucine-rich fragmenthad existed in the derived... |
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| Keywords: | Prunus salicina L cv Jiutaiwanli PGIP gene Gene cloning Bioinformatic Analysis |
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