Immunity to melanoma in mice immunized with transfected allogeneic mouse fibroblasts expressing melanoma-associated antigens |
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Authors: | Young Sang Kim Ryszard Slomski Edward P Cohen |
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Institution: | (1) Department of Microbiology and Immunology, University of Illinois College of Medicine, University of Illinois at Chicago, P. O. Box 6998, 60 680 Chicago, Illinois, USA;(2) Present address: Section in Immunobiology, Howard Hughes Medical Institute at Yale University School of Medicine, 06510 New Haven, Connecticut, USA;(3) Present address: Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland |
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Abstract: | Summary Transfection of genomic DNA from B16 mouse melanoma into LM(TK–) fibroblasts led to the generation of several clones of transfected cells that strongly expressed B 16 melanoma-associated antigens (MAA). The transfected cells retained their H-2k markers and served as allogeneic cells with expressive MAA in C57BL/6 mice, syngeneic with the melanoma. The cells were capable of eliciting primary anti-B16 immune responses in vitro in spleen cells from C57BL/6 mice. Immunization of C57BL/6 mice with the transfected cells led to the generation of anti-B16 cytotoxic activity in spleen cells, and C57BL/6 mice immunized with the MAA-positive transfected cells were partially resistant to a lethal challenge with B16 melanoma cells. Under similar conditions, B16 cells were nonimmunogenic. Therefore, transfected allogeneic LM(TK–) fibroblast cells expressing MAA served as more potent anti-melanoma immunogens than the parental B16 tumor cells themselves. |
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Keywords: | Melanoma Immunity Mouse fibroblasts Antigens |
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