Lack of evidence for an immunosuppressive role for MUC1 |
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Authors: | Stéphane Paul Nadine Bizouarne Annick Paul Michael R Price Gunnar C Hansson Marie Paule Kieny R Bruce Acres |
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Institution: | Department of Immunology, Transgène S. A, Strasbourg, France. |
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Abstract: | The in vitro anti-proliferative properties of various supernatants from MUC1-expressing cell lines and of purified preparations
of MUC1 were evaluated. We have observed that supernatants from the MUC1- and MUC3-positive cell line T47D, but not from the
MUC1- and MUC4-positive cell line MCF7, were able to inhibit proliferation of cells from various haematopoietic cell lines.
Although the activity of T47D supernatants could be abrogated by immunodepletion of MUC1, immunopurified MUC1 from T47D was
unable to inhibit cell proliferation. Significantly, supernatants from mouse 3T3 cells transfected with a secreted form of
MUC1 or from BHK-21 cells infected with a recombinant vaccinia virus coding for the secreted form of MUC1, as well as preparations
of purified MUC1 from bile or urine, were likewise unable to inhibit T cell proliferation. Surprisingly, a crude mixture of
bile mucins had a suppressive effect on T cell growth. Our results suggest that other molecules, such as amino sugars or other
mucins, which can associate with MUC1, are likely to be responsible for the observed anti-proliferative effects of T47D cells.
Received: 20 August 1998 / Accepted: 3 December 1998 |
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Keywords: | Tumour antigen MUC1 T cell immunosuppression Apoptosis |
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