| 植物原生质体的细胞壁再生 |
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| 引用本文： | 何其邹洪,鄂一岚,王广超,张贵芳,林金星,李瑞丽.植物原生质体的细胞壁再生[J].中国细胞生物学学报,2021(1). |
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| 作者姓名： | 何其邹洪 鄂一岚 王广超 张贵芳 林金星 李瑞丽 |
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| 作者单位： | 北京林业大学林木分子设计育种高精尖创新中心;北京林业大学生物科学与技术学院 |
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| 基金项目： | 国家自然科学基金(批准号:31970182、31670182、31530084、31761133009);中央高校基本科研业务费专项(批准号:2019ZY29);国家重点研发计划(批准号:2016YFD0600102)资助的课题。 |
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| 摘 要： | 细胞壁作为植物细胞重要的组成部分,在决定细胞形状、维持机械支撑、吸收养分等方面发挥重要功能。因此,揭示植物细胞壁合成的调控机制具有重大的生物学意义。基于植物组织水平研究细胞壁的生物合成具有难以控制时间尺度、观察空间狭小等局限性。原生质体作为去除细胞壁的单个细胞是研究细胞壁再生的理想系统。在过去的几十年里报道了大量关于植物原生质体再生细胞壁的研究,但是关于细胞壁再生的机制尚不清楚。该综述介绍了目前应用于植物原生质体再生细胞壁研究的主要技术和取得的研究进展,并且对该领域的后续发展进行了展望,为进一步阐明植物细胞壁生物合成的机制提供理论参考。
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| 关 键 词： | 植物 细胞壁再生 原生质体 细胞壁成像 原生质体培养 |
Cell Wall Regeneration of Plant Protoplast |
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| Authors: | HE Qizouhong E Yilan WANG Guangchao ZHANG Guifang LIN Jinxing LI Ruili |
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| Institution: | (Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,Beijing 100083,China;National Engineering Laboratory for Tree Breeding,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China) |
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| Abstract: | Cell wall,as a significant component of plant cell,is essential for determining cell shape,maintaining mechanical support,and absorbing nutrients of plants.Thus,it is of great biological significance to reveal the regulatory mechanism of plant cell wall biosynthesis.Studying cell wall biosynthesis based on organizational levels has great limitations in terms of controlling the time scale and narrow observation space.Protoplast is an ideal model system for studying cell wall regeneration with cell wall removed.Over the past few decades,a large number of studies on cell wall regeneration from plant protoplast have been reported.However the mechanism underlying this process is yet unclear.This review introduces the primary techniques and research progresses applied to the study of plant cell wall regeneration in protoplast and unveils a prospective advances of this field,providing theoretical reference for further clarifying the mechanism of cell wall biosynthesis. |
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| Keywords: | plant cell wall regeneration protoplast cell wall imaging protoplast culture |
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