| 人尿源性干细胞及人脐带间充质干细胞的生物学性状比较 |
| |
| 引用本文： | 胡超群,方姝煜,龚梦嘉,毕杨,何昀.人尿源性干细胞及人脐带间充质干细胞的生物学性状比较[J].中国细胞生物学学报,2020(2):239-247. |
| |
| 作者姓名： | 胡超群 方姝煜 龚梦嘉 毕杨 何昀 |
| |
| 作者单位： | 重庆医科大学附属儿童医院胃肠新生儿外科;重庆医科大学附属儿童医院儿研所干细胞实验室 |
| |
| 基金项目： | 重庆市科委基础研究与前沿探索项目(批准号:cstc2018jcyjAX0111)资助的课题。 |
| |
| 摘 要： | 该研究探讨人尿源性干细胞(human urine-derived stem cells,hUSCs)及人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,hUC-MSCs)的生物学性状差异。分离培养hUSCs及hUC-MSCs,显微镜下观察细胞形态,流式细胞术检测干细胞表面标记物,锥虫蓝拒染实验及克隆形成实验检测细胞增殖能力,划痕实验及Transwell迁移实验检测细胞迁移能力,碱性磷酸酶(alkaline phosphatase,ALP)染色、茜素红染色、油红O染色及阿利新蓝染色评估多向分化潜能。hUSCs为米粒状贴壁生长细胞,hUC-MSCs为长梭形贴壁细胞,呈旋涡状排列生长,两种细胞表型分析相似,均表达多种间充质干细胞标志物,但CD24在hUC-MSCs表达阳性,而CD105在hUSCs表达阳性。hUC-MSCs的增殖及迁移能力优于hUSCs,但后者的克隆形成能力更强。hUSCs及hUCMSCs都具有成骨、成脂、成软骨分化能力,hUC-MSCs的成骨能力强而hUSCs的成脂能力强。该研究成功分离培养出增殖能力强并具有多向分化潜能的hUSCs,该细胞与hUC-MSCs相比具有相似的生物学性状,可作为再生医学自体移植的理想种子细胞来源。
|
| 关 键 词： | 人尿源性干细胞 人脐带间充质干细胞 增殖 迁移 多向分化 |
Comparison of Biological Characteristics of Human Urine-Derived Stem Cells and Human Umbilical Cord Mesenchymal Stem Cells |
| |
| Authors: | HU Chaoqun FANG Shuyu GONG Mengjia BI Yang HE Yun |
| |
| Institution: | (Department of Neonatal Gastrointestinal Surgery,Ministry of Education Key Laboratory of Child Development and Disorders,National Clinical Research Center for Child Health and Disorders(Chongqing),China International Science and Technology Cooperation base of Child development and Critical Disorders,Children’s Hospital of Chongqing Medical University,Chongqing 400014,China;Stem Cell Biology and Therapy Laboratory,Chongqing Key Laboratory of Pediatrics,Children’s Hospital of Chongqing Medical University,Chongqing 400014,China) |
| |
| Abstract: | The aim of the research was to investigate and compare the biological characteristics of hUSCs(human urine-derived stem cells)and hUC-MSCs(human umbilical cord mesenchymal stem cells).hUSCs and hUCMSCs were isolated and cultured.The cell morphology was observed under microscope.Flow cytology was used to detect the stem cell surface markers.The proliferation ability was detected by trypan blue staining and colony formation assay.The migration ability was detected by wound-healing assay and transwell migration assay,ALP(alkaline phosphatase)staining,alizarin red staining,oil red O staining and alcian blue staining were performed to evaluate the multi-directional differentiation potential.hUSCs were rice-like adherent growth cells.hUC-MSCs were long spindle-shaped adherent cells which showed a spiral-like growth.The phenotypic analysis of the two cells was similar,both of the cells expressed multiple mesenchymal stem cell markers,but CD24 was positively expressed in hUC-MSCs while CD105 was positively expressed in hUSCs.The proliferation and migration ability of hUC-MSCs was superior to that of hUSCs,but the latter had stronger clonality.Both hUSCs and hUC-MSCs had the ability of osteogenic,adipogenic and chondrogenic differentiation.The osteogenesis ability of hUC-MSCs is stronger,while the adipogenic ability of hUSCs is stronger.We successfully isolated and cultured hUSCs with strong proliferative capacity and multi-directional differentiation potential.Compared with hUC-MSCs,hUSCs have similar biological characteristics and can be used as an ideal seed cell source for autologous transplantation in regenerative medicine. |
| |
| Keywords: | human urine-derived stem cells human umbilical cord mesenchymal stem cells proliferation migration multiple differentiation |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
