| 氰酸盐诱导氧化应激促进肾小管上皮细胞上皮–间充质转化 |
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| 引用本文： | 田宽,袁德智,胡玲,张滔,周鹏,郭星娴,陈益,郭小宇,李林颖,李静,冉建华.氰酸盐诱导氧化应激促进肾小管上皮细胞上皮–间充质转化[J].中国细胞生物学学报,2019(2):228-234. |
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| 作者姓名： | 田宽 袁德智 胡玲 张滔 周鹏 郭星娴 陈益 郭小宇 李林颖 李静 冉建华 |
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| 作者单位： | 重庆医科大学解剖教研室神经科学研究中心;重庆医科大学组织胚胎教研室组织细胞工程与干细胞研究室;重庆医科大学第二附属医院神经内科;重庆三峡医药高等专科学校 |
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| 基金项目： | 国家自然科学基金(批准号:81770738);重庆市科技计划项目(批准号:cstc2015jcyjA10036);重庆医科大学大学生项目(批准号:CXSY201802;CXSY201810)资助的课题~~ |
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| 摘 要： | 该研究探讨氰酸盐(cyanate)诱导肾小管上皮细胞氧化应激损伤和促进肾纤维化的作用。氰酸盐作用HK-2肾小管上皮细胞后, CCK8法检测其对细胞活力的影响;倒置显微镜观察细胞形态的改变; DCFH-DA法检测细胞ROS水平;细胞免疫荧光和Western blot分别检测E-cadherin、Fibronectin、α-SMA的表达; Western blot检测TGF-β的表达水平。结果显示, 2 mmol/L氰酸盐明显下调HK-2细胞的活力(P<0.05),细胞形态变为长梭形。氰酸盐作用24 h后, HK-2细胞内ROS水平呈浓度依赖性升高。免疫荧光和Western blot结果均显示,氰酸盐作用24 h后, HK-2的Fibronectin、α-SMA表达升高, E-cadherin表达下降; TGF-β的表达水平随氰酸盐浓度升高而上调(P<0.05)。以上结果表明,氰酸盐诱导肾小管上皮细胞产生过量ROS,上调TGF-β水平促进细胞上皮–间充质细胞转化(epithelia-mesenchymal transition, EMT)。
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| 关 键 词： | 氰酸盐 HK-2细胞 氧化应激 上皮–间充质细胞转化 |
Cyanate Induces Oxidative Stress and Epithelia-Mesenchymal Transition on Renal Tubular Epithelial Cells |
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| Authors: | Tian Kuan Yuan Dezhi Hu Ling Zhang Tao Zhou Peng Guo Xingxian Chen Yi Guo Xiaoyu Li Linying Li Jing Ran Jianhua |
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| Institution: | (Center of Neuroscience Research,Department of Anatomy,Chongqing Medical University,Chongqing 400016,China;Department of Histology and Stem Cell Research,Department of Histology and Embryology,Chongqing Medical University,Chongqing 400016, China;Department of Neurology,the Second Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China;Chongqing Three Gorges Medical College,Chongqing 404120,China) |
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| Abstract: | Tian Kuan;Yuan Dezhi;Hu Ling;Zhang Tao;Zhou Peng;Guo Xingxian;Chen Yi;Guo Xiaoyu;Li Linying;Li Jing;Ran Jianhua(Center of Neuroscience Research,Department of Anatomy,Chongqing Medical University,Chongqing 400016,China;Department of Histology and Stem Cell Research,Department of Histology and Embryology,Chongqing Medical University,Chongqing 400016, China;Department of Neurology,the Second Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China;Chongqing Three Gorges Medical College,Chongqing 404120,China) |
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| Keywords: | cyanate HK-2 oxidative stress epithelia-mesenchymal transition |
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