Age- and gender-related oxidative status determined in healthy subjects by means of OXY-SCORE,a potential new comprehensive index |
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Authors: | F Veglia G Cighetti M De Franceschi L Zingaro L Boccotti E Tremoli |
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Institution: | 1. Centro Cardiologico Monzino I.R.C.C.S., Milan, Italy;2. Department of Preclinical Sciences, LITA, Vialba, Italy;3. Institute of Cardiology, University of Milan, Milan, Italy;4. Department of Pharmacological Sciences, University of Milan, Milan, Italy |
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Abstract: | Oxidative stress has been related to various diseases, gender and ageing, and has been measured by various markers. The authors developed a procedure to compute a global oxidative stress index (OXY-SCORE), reflecting both oxidative and antioxidant markers in healthy subjects. Its performance was tested in relation to age and gender and in coronary artery disease (CAD) patients. Eighty-two healthy subjects and 20 CAD patients were enrolled. Plasma free and total malondialdehyde (F- and T-MDA), glutathione disulphide/reduced form ratio (GSSG/GSH) and urine isoprostanes (iPF2α-III) levels were combined as oxidative damage markers (damage score). GSH, α- and γ-tocopherol (TH) levels, and individual antioxidant capacity were combined as antioxidant defence indexes (protection score). The OXY-SCORE was computed by subtracting the protection score from the damage score. Among single parameters, T-MDA and iPF2α-III significantly correlated with age; only GSH and both tocopherols correlated with male gender in healthy subjects. The OXY-SCORE was positively associated with age (p=0.004) and male gender (p=0.03). As expected, the OXY-SCORE was higher in CAD with a very significant p-value (<0.0001), after adjusting for age, gender and smoking. Combining different markers can potentially provide a powerful index in the evaluation of oxidative stress related to age, gender and CAD status. |
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Keywords: | Oxidative stress markers pro/antioxidant global indices ageing coronary artery disease (CAD) |
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