Identification of a new V kappa gene family that is highly expressed in hybridomas from an autoimmune mouse strain |
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Authors: | R Shefner R Mayer A Kaushik P D'Eustachio C Bona B Diamond |
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Institution: | Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461. |
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Abstract: | We have identified a new murine V kappa family that contains five to seven members, one member of which encodes the L chain V region of an anti-dsDNA antibody produced by a BALB/c hybridoma, C8.5. The cloned C8.5 V kappa gene exhibits highest homology with a human V kappa gene that was cloned from a nonproductive rearrangement but has never been seen in an expressed repertoire. Because this family was first identified in an autoantibody, we studied its expression in an autoimmune mouse strain. This V kappa family is expressed in 20% of hybridomas from NZB mice. |
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