Refined genetic and comparative physical mapping of the canine copper toxicosis locus |
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| Authors: | Bart van de Sluis Susan Kole Monique van Wolferen Nigel G Holmes Peter L Pearson Jan Rothuizen Bernard A van Oost Cisca Wijmenga |
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| Institution: | (1) Department of Medical Genetics, KC04.084.2, University Medical Center Utrecht, WKZ, Lundlaan 6, 3584 EA Utrecht, the Netherlands, NL;(2) Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, the Netherlands, NL;(3) Centre for Preventive Medicine, Animal Health Trust, Suffolk, UK, GB |
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| Abstract: | Recently, the copper toxicosis (CT) locus in Bedlington terriers was assigned to canine chromosome region CFA10q26, which is homologous to human chromosome
region HSA2p13-21. A comparative map between CFA10q21-26 and HSA2p13-21 was constructed by using genes already localized to
HSA2p13-21. A high-resolution radiation map of CFA10q21-26 was constructed to facilitate positional cloning of the CT gene. For this map, seven Type I and eleven Type II markers were mapped. Using homozygosity mapping, the CT locus could be confined to a 42.3 cR3000 region, between the FH2523 and C10.602 markers. On the basis of a partial BAC contig, it was estimated that 1-cR3000 is equivalent to approximately 210 kb, implying that the CT candidate region is therefore estimated to be about 9 Mb.
Received: 16 December 1999 / Accepted: 23 February 2000 |
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