Detection of quantitative trait loci for body weight at 10 weeks from Philippine wild mice |
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Authors: | Akira Ishikawa Yoichi Matsuda Takao Namikawa |
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Institution: | (1) Laboratory of Animal Genetics, Division of Applied Genetics and Physiology, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan, JP |
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Abstract: | A genome-wide scan for quantitative trait loci (QTLs) controlling body weight at 10 weeks after birth was carried out in
a population of 387 intersubspecific backcross mice derived from a cross between C57BL/6J inbred mice (Mus musculus domesticus) and wild mice (M. m. castaneus) captured in the Philippines, in order to discover novel QTLs from the wild mice that have about 60% lower body weight than
C57BL/6J. By interval mapping, we detected four QTLs: a highly significant QTL on Chromosome (Chr) 2, which was common in
both sexes; two significant QTLs on Chr 13, one male-specific and the other female-specific; and a suggestive male-specific
QTL on X Chr. By composite interval mapping, we confirmed the presence of the three QTLs on Chrs 2 and 13, but not of the
male-specific X-linked QTL. The composite interval mapping analysis newly identified three QTLs: a significant male-specific
QTL on Chr 11 and two highly significant female-specific QTLs on Chrs 9 and X. Individual QTLs explained 3.8–11.6% of the
phenotypic variance, and all the QTL alleles derived from the wild mice decreased body weight. A two-way analysis of variance
revealed a significant epistatic interaction between the Chr 2 QTL and the background marker locus D12Mit4 on Chr 12 only in males. The interaction effect unexpectedly increased body weight. The chromosomal region containing the
Chr 2 QTL did not coincide with those of growth or fatness QTLs mapped in previous studies. These results suggest that a population
of wild mice may play an important role as new sources of valuable QTLs.
Received: 14 January 2000 / Accepted: 14 April 2000 |
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