Uptake of selenium-75 by PHA-stimulated lymphocytes |
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Authors: | Judy A Karle Fredrick J Kull Alex Shrift |
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Institution: | 1. Department of Biological Sciences, State University of New York, 13901, Binghamton, NY
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Abstract: | To determine which of a variety of inorganic and organic selenium compounds could best stimulate glutathione peroxidase, human lymphocytes were cultured with a number of selenium sources. The phytohemagglutinin-transformed lymphocytes were cultured in the presence of75Se bound to serum proteins (25% v/v) or 10?7 M concentrations of 75Se]-selenite, 75Se]-selenate, 75Se]-selenocystine, and 75Se]-selenomethionine. Organic forms of selenium were taken up in preference to inorganic forms. Control cultures, from which exogenous selenium had been omitted, showed a decreased level of glutathione peroxidase activity at the end of a 4 d culture period. Of the Se sources tested, 75Se]-selenocystine and 75Se]-labeled fetal calf serum proteins increased enzyme activity significantly, 79 and 47%, respectively, but selenite increased activity only by 7%. These results indicate that selenium from the two organic sources is most readily available for glutathione peroxidase synthesis. |
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