The mechanism for the effect of selenium supplementation on immunity |
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Authors: | Enjie Sun Huibi Xu Qiong Liu Jingyan Zhou Ping Zuo Junjian Wang |
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Institution: | (1) Department of Chemistry, Huazhong University of Science and Technology, 430074 Wuhan, Peoples Republic of China;(2) Department of Biological and Medical Engineering, Huazhong University of Science and Technology, 430074 Wuhan, Peoples Republic of China |
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Abstract: | Lipid peroxide (LPO) in lymphocytes from mice was evaluated by measuring substances reactive to thiobarbituric acid (TBA).
The product resulting from the reaction of TBA with lymphocytes was extracted with n-butyl and fluorescence intensity was
determined. The degree of lipid peroxidation, expressed as fluorescence intensity f547, was assessed for stimulation of lymphocytes
with concanavalin A (Con A), and was related to lymphocyte proliferation in response to Con A if Se was administered. The
lymphocyte proliferation was determined by 3H]thymidine incorporation, expressed as cpm. The effect of superoxide dismutase (SOD), added to cell culture on lymphocyte
proliferation was also evaluated. It was found that LPO in lymphocytes before Con A stimulation was significantly less than
that after stimulation (p<0.001), and that SOD promoted lymphocyte proliferation dose dependently. The addition of Na2SeO3 to lymphocyte culture or supplementation in drinking water to mice decreased the produced LPO in lymphocyte in response to
Con A. In the presence of Se, there is an inverse correlation between the levels of LPO in lymphocyte and the stimulated proliferation
(r=−0.8902,r=−0.9439). In conclusion, active oxygen species scavenging was proposed as one of the mechanisms for Se to promote immunity. |
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Keywords: | Selenium supplementation immunity thiobarbituric acid (TBA) |
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