Cytoprotection against lipid hydroperoxides correlates with increased glutathione peroxidase activities, but not selenium uptake from different selenocompounds |
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Authors: | Marcel Leist Stefanie Maurer Manfred Schultz Angelika Elsner Dieter Gawlik Regina Brigelius-Flohé |
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Institution: | (1) German Institute of Human Nutrition, Arthur-Scheunert Allee, 114-116 Potsdam-Rehbrücke, Germany;(2) Hahn-Meitner Institute, Berlin, Germany |
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Abstract: | Cells cultivated under standard conditions were highly deficient in tocopherol, selenium, and glutathione peroxidase (GPx)
activities. We investigated whether and to what extent the addition of different selenocompounds to growth media would alter
biochemical, physiological, and pathophysiological parameters of cultured liver cells. Cellular uptake of selenium, GPx activities,
and cytoprotection were measured and compared in human hepatoma cells (HepG2). Selenite and selenocystine were Se donors of
high bioavailability (i.e., with these culture supplements, the increased Se uptake, induction of GPx isoenzymes, and protection
of treated cells from lipid hydroperoxides were well correlated). In contrast, selenium from selenomethionine was incorporated
into cellular proteins but had no effect on GPx activities or cytoprotection. The data show that not all selenium donors provide
selenium, which is bioactivated to act as antioxidant. Thus, cellular selenium content, in general, did not correlate with
cytoprotective activity of this trace element. However, cellular GPx activities at different times, with different concentrations,
and with different Se donors always correlated with protection from lipid hydroperoxides and may, thus, represent a more reliable
parameter to define adequate Se supply. |
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Keywords: | Selenium cell culture media glutathione peroxidase fatty acid hydroperoxide oxidative stress |
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