Iron,Copper, and Zinc Transport: Inhibition of Divalent Metal Transporter 1 (DMT1) and Human Copper Transporter 1 (hCTR1) by shRNA |
| |
Authors: | Alejandra Espinoza Solange Le Blanc Manuel Olivares Fernando Pizarro Manuel Ruz Miguel Arredondo |
| |
Institution: | 1.Micronutrient Laboratory, Institute of Nutrition and Food Technology,University of Chile,Macul,Chile;2.Department of Nutrition, Faculty of Medicine,University of Chile,Santiago,Chile |
| |
Abstract: | Iron (Fe), copper (Cu), and zinc (Zn) fulfill various essential biological functions and are vital for all living organisms.
They play important roles in oxygen transport, cell growth and differentiation, neurotransmitter synthesis, myelination, and
synaptic transmission. Because of their role in many critical functions, they are commonly used in food fortification and
supplementation strategies globally. To determine the involvement of divalent metal transporter 1 (DMT1) and human copper
transporter 1 (hCTR1) on Fe, Cu, and Zn uptake, Caco-2 cells were transfected with four different shRNA plasmids to selectively
inhibit DMT1 or hCTR1 transporter expression. Fe and Cu uptake and total Zn content measurements were performed in shRNA-DMT1
and shRNA-hCTR1 cells. Both shRNA-DMT1 and shRNA-hCTR1 cells had lower apical Fe uptake (a decrease of 51% and 41%, respectively),
Cu uptake (a decrease of 25.8% and 38.5%, respectively), and Zn content (a decrease of 23.1% and 22.7%, respectively) compared
to control cells. These results confirm that DMT1 is involved in active transport of Fe, Cu, and Zn although Zn showed a different
relative capacity. These results also show that hCTR1 is able to transport Fe and Zn. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|