Chromium Improves Protein Deposition Through Regulating the mRNA Levels of IGF-1, IGF-1R,and Ub in Rat Skeletal Muscle Cells |
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Authors: | Zhongli Peng Wei Qiao Zhisheng Wang Qiuzhong Dai Jianhua He Chunhua Guo Jun Xu Anguo Zhou |
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Institution: | (1) Engineering Research Center of Animal Disease Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an, 625014, China;(2) Hunan Institute of Animal Science and Veterinary Medicine, Changsha, 410131, China;(3) Hunan Agricultural University, Changsha, 410128, China;(4) Southwest University for Nationalities, Chengdu, 610041, China;(5) Chengdu Vocational College of Agricultural Science and Technology, Chengdu, 611130, China; |
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Abstract: | The aim of this study was to evaluate the impact of three different chromium forms—chromic chloride (CrCl3), chromium picolinate (CrPic), and a newly synthesized complex of chromium chelated with small peptides (CrSP)—on protein
metabolism in vitro. In cultured skeletal muscle cells, CrSP was able to increase the basal and insulin-stimulated levels
of protein deposition in skeletal muscles cells. CrCl3 and CrPic augmented insulin-stimulated protein synthesis. At the molecular level, insulin significantly increased the mRNA
levels of insulin-like growth factor 1 and insulin-like growth factor 1 receptor. These impacts could be enhanced by the addition
of chromium, especially CrSP. The mRNA levels of ubiquitin were significantly reduced when cells were cultured with chromium
or/and insulin. Assuming that the mRNA level increase or decrease results in increased or decreased levels of these proteins,
chromium would improve protein anabolism and reduce protein catabolism and then prove protein deposition in rat skeletal muscle
cells. |
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