Technique of the Modulated Chlorophyll Fluorescence: Basic Concepts,Useful Parameters,and Some Applications |
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| Authors: | Roháček K Barták M |
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| Institution: | (1) Department of Plant Physiology and Anatomy, Masaryk University, Kotlářská 2, CZ-61137 Brno, Czech Republic |
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| Abstract: | The review summarizes basic information about slow and fast chlorophyll (Chl) a fluorescence induction kinetics (FIK) recorded using fluorimeters working on a principle of the pulse amplitude modulation
(PAM) of a Chl fluorescence signal. It explains fundamental principles of the measuring technique, evaluates the terminology,
symbols, and parameters used. Analysis of Chl FIK resulting in a set of Chl fluorescence parameters (FPs) provides qualitative
and quantitative information about photosynthetic processes in chloroplasts. Using FPs, one can describe the functioning of
the photosynthetic apparatus under different internal and external conditions. Brief comments on proper application of the
fluorimetric method in photosynthesis research and some actual examples are also given.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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| Keywords: | chemicals circadien cycle dark-adapted state fast kinetics fluoranthene light-adapted state photosystem 2 slow kinetics temperature |
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