高粱LEA基因家族的鉴定及表达分析

有研究表明，干旱、低温和盐等环境胁迫能够诱导LEA基因的表达。为了探索LEA基因家族在高粱响应外界刺激过程中起到的作用，本研究通过生物信息学的方法对LEA基因家族在高粱全基因组水平进行鉴定和分析，于高粱全基因组中共鉴定出35个基因家族成员，不均匀地分布于高粱8条染色体上，结合系统进化树和保守结构域分析结果，将高粱LEA基因家族成员分为7组。亲水性分析和结构无序性预测表明高粱LEA蛋白绝大多数为亲水性且结构无序。基因结构分析显示了各分组基因结构上的保守性。高粱LEA基因的启动子分析发现了一些与激素和非生物胁迫响应相关的顺式作用元件。对激素和干旱胁迫下高粱LEA基因的表达分析发现外界胁迫能够诱导部分高粱LEA基因的表达。

Genome-wide identification and expression analysis of LEA gene family in Sorghum bicolor L.

A large number of studies have indicated that environmental stresses such as drought, low temperature and salt can induce the expression of LEA gene. In order to explore their roles in response to external stimuli in sorghum, LEA gene family was identified and analyzed by bioinformatics at the sorghum genome level. A total of 35 LEA gene family members were found in the sorghum genome, which were unevenly distributed on 8 chromosomes of sorghum. According to the results of phylogenetic tree and conserved domain analysis, the sorghum LEA gene family members were divided into 7 groups.Hydrophilic analysis and structural disorder prediction indicated that most of the sorghum LEA proteins were hydrophilic and structurally disordered. Gene structure analysis revealed structural conservation of each grouped gene. Promoter analysis found some cis-acting elements associated with hormonal and abiotic stress. The expression analysis of LEA gene under hormone and drought stress showed that external stress could induce the expression of some LEA genes.