| 钙调神经磷酸酶在血管紧张素Ⅱ刺激的心脏成纤维细胞增殖中的作用 |
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| 作者姓名： | Xu S Fu MG Xu YF Pang YZ Tang CS |
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| 作者单位： | 1. 北京医科大学第一医院中心实验室,北京,100034
2. 北京医科大学第一医院心血管研究所,北京,100034 |
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| 基金项目： | SupportedbytheNationalNaturalScienceFoundationofChina (No 39730 2 2 0 ) |
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| 摘 要： | 本研究观察了钙调神经磷酸酶（ＣａＮ）在血管坚张素Ⅱ（ＡｎｇⅡ）刺激的大鼠心脏成纤维细胞增殖中的作用。在培养的大鼠心脏成纤维细胞上,应用双波长荧光 计检测Ｆｕｒａ－２标记的细胞游离Ｃａ＾２＋浓度;应用对硝基苯磷酸（ＰＮＰＰ）作底物测定钙调神经磷酸酶（ＣａＮ）活性;根据＾３Ｈ－胸腺嘧啶掺入法评估ＣａＮ特异性抑制剂环胞素Ａ（ＣｓＡ）对ＡｎｇⅡ刺激的心脏成纤维细胞ＤＮＡ合成的影响。结果表明,ＡｎｇⅡ（１０
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| 关 键 词： | 钙调神经磷酸酶 血管紧张素Ⅱ 成纤维细胞增殖 |
Role of calcineurin in cardiac fibroblast proliferation stimulated by angiotensin II |
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| Xu S,Fu MG,Xu YF,Pang YZ,Tang CS.Role of calcineurin in cardiac fibroblast proliferation stimulated by angiotensin II[J].Acta Physiologica Sinica,2000,52(4):305-307. |
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| Authors: | Xu S Fu M G Xu Y F Pang Y Z Tang C S |
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| Institution: | Institute of Cardiovascular Research, the First Hospital, Beijing Medical University, Beijing 100034, China. tangchaoshu@263.net |
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| Abstract: | The present study was undertaken to observe the role of calcineurin (CaN) in the angiotensin II (Ang II) stimulated cardiac fibroblast proliferation. In cultured cardiac fibroblasts of neonatal rats, Ang II was used to stimulate proliferation while CaN was inhibited by CaN CsA inhibitor cyclosporin A (CsA). (3)H-TdR incorporation, activity of CaN and intracellular calcium concentration were measured. (3)H-TdR incorporation of Ang II stimulated fibroblasts was 72% higher than control (P<0.01), which was inhibited by CsA (0.1 10 micromol/L) in a dose dependent manner. Intracellular Ca(2+) level and CaN activity of Ang II stimulated fibroblasts were respectively 112% (P<0.01) and 17%(P<0.05) higher than control. It is concluded that CaN may play an important role in signal transduction of the Ang II induced cardiac fibroblast proliferation. |
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