乙酰胆碱对培养T细胞功能的作用

本文研究不同浓度（１０＾－１０－１０＾－４ｍｏｌ／Ｌ）乙酰胆碱（ＡＣｈ）对离体培养的大鼠脾脏Ｔ淋巴细胞增殖的影响，并探讨其作用机制。实验结果表明：１０＾－９－１０＾－４ｍｏｌ／Ｌ可显著增强Ｔ细胞由刀豆素Ａ（Ｃ－Ａ）诱导的增殖反应，以１０＾－７－１０＾－６ｍｏｌ／Ｌ时最强。淋巴细胞先用ＡＣｈ刺激１ｈ或者刺激１ｈ后洗弃ＡＣｈ，再用ＣｏｎＡ诱导６ｈ的Ｔ细胞的增殖。１０＾－７－１０－６ｍｏｌ／Ｌ阿托品可阻

EFFECT OF ACETYLCHOLINE ON THE PROLIFERATION OF T LYMPHOCYTE OF RAT SPLEEN

Experiments were performed to determine the effect of various concentrations of acetylcholine (ACh) (10-10 to 10-4 mol/L) on the proliferation of spleen cells of rat to concanavalin (Con A). ACh at 10-9 mol/L to 10-4 mol/L range significantly enhanced the proliferation of T cells, reaching a maximum at 10-7 and 10-6 mol/L. The proliferation of lymphocytes first stimulated by various concentrations of ACh (10-9,10-v or 10-5 mol/L) for one hour and incubated with Con A for 72 h still showed increase. When ACh was washed off for one hour, the proliferative enhancement of lymphocytes to Con A remained to occur. However, ACh did not enhance the proliferation of T cells which were once stimulated with Con A for 6 h. Atropine at 10-7 or 10-6 mol/L abolished completely the effect of ACh at 10-9 or 10-5 mol/L. The action of ACh at 10-7 mol/L could be blocked by 10-6 mol/L atropine. These daal suggest that ACh at a wide range of concentrations can enhance cellular immunity, an effect occurring either before or after T cells are activated through muscarinic cholinergic receptors.